التفاصيل البيبلوغرافية
| العنوان: |
GPR80/99, proposed to be the P2Y 15 receptor activated by adenosine and AMP, is not a P2Y receptor |
| المؤلفون: |
Ai-dong Qi, T. Kendall Harden, Robert A. Nicholas |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/b9/31/Purinergic_Signal_2004_Dec_1(1)_67-74.tar.gz |
| سنة النشر: |
2004 |
| المجموعة: |
CiteSeerX |
| مصطلحات موضوعية: |
4S-disulphonic acid, RIA – radioimmunoassay |
| الوصف: |
receptor The orphan receptor GPR80 (also called GPR99) was recently reported to be the P2Y15 receptor activated by AMP and adenosine and coupled to increases in cyclic AMP accumulation and intracellular Ca 2+ mobilization (Inbe et al. J Biol Chem 2004; 279: 19790–9 [12]). However, the cell line (HEK293) used to carry out those studies endogenously expresses A2A and A2B adenosine receptors as well as multiple P2Y receptors, which complicates the analysis of a potential P2Y receptor. To determine unambiguously whether GPR80 is a P2Y receptor subtype, HA-tagged GPR80 was either stably expressed in CHO cells or transiently expressed in COS-7 and HEK293 cells, and cell surface expression was verified by radioimmunoassay (RIA). COS-7 cells overexpressing GPR80 showed a consistent twofold increase in basal inositol phosphate accumulation. However, neither adenosine nor AMP was capable of promoting accumulation of either cyclic AMP or inositol phosphates in any of the three GPR80-expressing cells. A recent paper (He et al. Nature 2004; 429: 188–93 [15]) reported that GPR80 is a Gq-coupled receptor activated by the citric acid cycle intermediate, a-ketoglutarate. Consistent with this report, a-ketoglutarate promoted inositol phosphate accumulation in CHO and HEK293 cells expressing GPR80, and pretreatment of GPR80-expressing COS-7 cells with glutamate dehydrogenase, which converts a-ketoglutarate to glutamate, decreased basal levels of inositol phosphates. Taken together, these data |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/zip |
| اللغة: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.283.8476 |
| الاتاحة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.283.8476 |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.3C856093 |
| قاعدة البيانات: |
BASE |