التفاصيل البيبلوغرافية
| العنوان: |
daf -5 is not required to block col-19::gfp expression during dauer. |
| المؤلفون: |
Matthew J. Wirick (11708043), Allison R. Cale (11708046), Isaac T. Smith (11708049), Amelia F. Alessi (11708052), Margaret R. Starostik (11708055), Liberta Cuko (11708058), Kyal Lalk (11708061), Mikayla N. Schmidt (11708064), Benjamin S. Olson (9629251), Payton M. Salomon (11708067), Alexis Santos (11708070), Axel Schmitter-Sánchez (11708073), Himani Galagali (11708076), Kevin J. Ranke (11708079), Payton A. Wolbert (11708082), Macy L. Knoblock (11708085), John K. Kim (8575362), Xantha Karp (8729403) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Cell Biology, Genetics, Molecular Biology, Developmental Biology, Cancer, Infectious Diseases, Computational Biology, prematurely adopting differentiated, multipotent cell fate, misexpression phenotype observed, div >< p, binding protein orthologous, adverse environmental cues, single foxo ortholog, progenitor cells maintain, allow normal development, adult cell fate, stem cell maintenance, heterochronic gene network, caenorhabditis elegans <, dauer larvae via, >, promotes entry, heterochronic gene, elegans <, seam cells, dauer development, >/ foxo, normally adult, adult characteristics |
| الوصف: |
The daf- 7/TGFβ pathway regulates dauer formation in parallel to the insulin-like pathway [ 6 , 7 ]. daf-5 encodes a Sno/Ski protein that works in a complex with DAF-3/SMAD to regulate transcription downstream of DAF-7/TGFβ signaling [ 76 ]. daf-5(0) mutants are dauer-defective, but can be induced to enter dauer when combined with the daf-2(e1370) allele [ 34 , 35 ]. (A) daf-2(e1370) mutants develop slowly and acquire dauer characteristics approximately one day later than wild-type or daf-7(e1372) larvae [ 60 , 77 ]. Since daf-16; daf-7 dauer larvae express col-19p :: gfp most penetrantly within one day of dauer entry, we used SDS-resistance to determine a time that might correspond to “early dauer” in this strain. SDS-resistance is acquired at the end of the L2d-to-dauer molt and continues throughout dauer [ 2 ]. Gravid adult hermaphrodites of the indicated strains were allowed to lay eggs for 3 hours and then removed. Embryos were incubated at 24°C for the indicated times and then incubated in 1% SDS for 10 minutes. The percent of larvae alive after this treatment is shown; data were aggregated from four independent experiments. (B) Percent of dauer larvae expressing col-19p :: gfp . This expression was never observed in either control or daf-5(0) dauer larvae at times when many early dauer larvae should have been present. Data shown were aggregated from 3–4 independent experiments. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/_i_daf_i_-5_is_not_required_to_block_i_col-19_gfp_i_expression_during_dauer_/17017716 |
| DOI: |
10.1371/journal.pgen.1009881.s001 |
| الاتاحة: |
https://doi.org/10.1371/journal.pgen.1009881.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.3C372F30 |
| قاعدة البيانات: |
BASE |