Academic Journal
Protein Ligation and Labeling Enabled by a C‐Terminal Tetracysteine Tag
| العنوان: | Protein Ligation and Labeling Enabled by a C‐Terminal Tetracysteine Tag |
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| المؤلفون: | Mo, Zeyuan, Lin, Shaomin, Chen, Wentao, He, Chunmao |
| المساهمون: | National Natural Science Foundation of China, Fundamental Research Funds for the Central Universities |
| المصدر: | Angewandte Chemie International Edition ; volume 61, issue 15 ; ISSN 1433-7851 1521-3773 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2022 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | The hydrazinolysis of S‐cyanylated peptide provides an alternative way to afford protein α‐hydrazide, a key reagent used in native chemical ligation (NCL), without the aid of any inteins or enzymes. The currently used non‐selective S‐cyanylation, however, allows no other cysteine in the protein besides the one at the cleavage site. Herein, we report a regioselective S‐cyanylation and hydrazinolysis strategy achieved via the fusion of a tetracysteine tag to the C‐terminal of the protein of interest. We term it tetracysteine enabled protein ligation (TCEPL). While highly selective, the strategy is applicable for proteins expressed as inclusion bodies, and this was showcased by the efficient semi‐synthesis of an iron‐sulfur protein rubredoxin and the catalytic and hinge domains of matrix metalloprotease‐14 (MMP‐14) containing 207 amino acid residues. Furthermore, the TCEPL strategy was exploited for protein C‐terminal labeling with amino reagents bearing a variety of functional groups, demonstrating its versatility and generality. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/anie.202115377 |
| الاتاحة: | http://dx.doi.org/10.1002/anie.202115377 https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.202115377 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/anie.202115377 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.3BF8513A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/anie.202115377 |
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