Academic Journal
Rapid determination of nucleotides in infant formula by means of nano‐liquid chromatography
| العنوان: | Rapid determination of nucleotides in infant formula by means of nano‐liquid chromatography |
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| المؤلفون: | Mateos‐Vivas, María, Fanali, Salvatore, Rodríguez‐Gonzalo, Encarnacíon, Carabias‐Martínez, Rita, Aturki, Zeineb |
| المصدر: | ELECTROPHORESIS ; volume 37, issue 13, page 1873-1880 ; ISSN 0173-0835 1522-2683 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2016 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | A rapid method for the quantification of five ribonucleotides 5′‐ monophophates (adenosine, cytidine, guanosine, inosine, uridine, 5′‐monophosphate), in infant formula, has been proposed using nano‐LC. To separate the studied compounds, capillary columns packed with different C18‐based stationary phases were investigated. All the columns tested were laboratory prepared. The experiments were performed in ion‐pairing RP chromatographic mode using tetrabutylammonium hydroxide as ion‐pairing reagent. The method was developed using a core‐shell XB‐C 18 capillary column with a mobile phase consisting of 5% v/v methanol and 95% v/v 100 mM ammonium formate, pH 8, containing 20 mM tetrabutylammonium hydroxide. All compounds were baseline resolved in less than 5 min with a flow rate of 500 nL/min in isocratic elution mode. Nucleotides were detected at 260 nm. Analytical validation parameters were evaluated. The RSD values for intraday and interday repeatability for retention time and peak area were <2.4 and 4.2%, respectively. The method linearity was good ( R 2 < 0.9995) for the studied compounds. LOD and limit of quantitation were 0.25 and 0.50 μg/mL, respectively. The method was applied to the determination of nucleotides in infant formula, subjected to a centrifugal ultrafiltration process, prior their analysis. The amounts found were in agreement to the labeled contents. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/elps.201500526 |
| DOI: | 10.1002/elps.201500526/fullpdf |
| الاتاحة: | http://dx.doi.org/10.1002/elps.201500526 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Felps.201500526 http://onlinelibrary.wiley.com/wol1/doi/10.1002/elps.201500526/fullpdf |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.3BC08BE7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/elps.201500526 |
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