Academic Journal
Estimating Individual Contributions to Complex DNA SNP Mixtures.
| العنوان: | Estimating Individual Contributions to Complex DNA SNP Mixtures. |
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| المؤلفون: | Ricke, Darrell O., Fremont‐Smith, Philip, Watkins, James, Stankiewicz, Sara, Boettcher, Tara, Schwoebel, Eric |
| المساهمون: | U.S. Air Force |
| المصدر: | Journal of Forensic Sciences ; volume 64, issue 5, page 1468-1474 ; ISSN 0022-1198 1556-4029 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2019 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | High‐throughput sequencing ( HTS ) of large panels of single nucleotide polymorphisms ( SNP s) provides an alternative or complimentary approach to short tandem repeats ( STR s) panels for the analysis of complex DNA mixture forensic samples. For STR s, methods to estimate individual contribution concentrations compare capillary electrophoresis peak heights, peak areas, or HTS allele read counts within a mixture. This article introduces three approaches (mean, median, and slope methods) for estimating individual DNA contributions to forensic mixtures for HTS /massively parallel sequencing ( MPS ) SNP panels. For SNP s, the major:minor allele ratios or counts, unique to each contributor, were compared to estimate contributor proportion within the mixture using the mean, median, and slope intercept for these alleles. The estimates for these three methods were typically within 5% of planned experimental contributions for defined mixtures. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/1556-4029.14030 |
| الاتاحة: | http://dx.doi.org/10.1111/1556-4029.14030 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1556-4029.14030 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1556-4029.14030 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1556-4029.14030 https://onlinelibrary.wiley.com/doi/am-pdf/10.1111/1556-4029.14030 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#am ; http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.3B5E245C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/1556-4029.14030 |
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