Academic Journal
Electrostatic Effects Influence the Formation of Two-Dimensional Crystals of Bacteriorhodopsin Reconstituted into Dimyristoylphosphatidylcholine Membranes
| العنوان: | Electrostatic Effects Influence the Formation of Two-Dimensional Crystals of Bacteriorhodopsin Reconstituted into Dimyristoylphosphatidylcholine Membranes |
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| المؤلفون: | Negishi, Lumi, Mitaku, Shigeki |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2011 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Regular Paper |
| الوصف: | We examined how electrostatic shielding affects the formation of two-dimensional (2D) crystals of bacteriorhodopsin (bR) in reconstituted dimyristoylphosphatidylcholine (DMPC) membranes by varying the sodium chloride (NaCl) concentration. The 2D crystalline array of bR formed in the gel phase of DMPC membranes was characterized by a symmetric bipolar pattern in visible circular dichroic spectra collected around 560 nm. The amplitude of the bipolar pattern was systematically enhanced by increasing the NaCl concentration. A strong correlation between the amplitude of the bipolar pattern and the Debye constant of small ions indicated that a weakening of electrostatic repulsion by the shielding effect of small ions enhances the order of 2D bR crystals in the gel phase of DMPC membranes. Considering the three-dimensional distribution of charged residues, we propose a model of interaction balance in reconstituted bR membranes in which effective attraction between bR molecules occurs as a result of the phase separation of the DMPC membrane in the gel phase overcoming electrostatic repulsion between bR molecules. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://jb.oxfordjournals.org/cgi/content/short/mvr043v1; http://dx.doi.org/10.1093/jb/mvr043 |
| DOI: | 10.1093/jb/mvr043 |
| الاتاحة: | http://jb.oxfordjournals.org/cgi/content/short/mvr043v1 https://doi.org/10.1093/jb/mvr043 |
| Rights: | Copyright (C) 2011, Japanese Biochemical Society |
| رقم الانضمام: | edsbas.3B486DC0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/jb/mvr043 |
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