Academic Journal
HIV-1 Vpr reactivates latent HIV-1 provirus by inducing depletion of class I HDACs on chromatin
| العنوان: | HIV-1 Vpr reactivates latent HIV-1 provirus by inducing depletion of class I HDACs on chromatin |
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| المؤلفون: | Romani, Bizhan, Kamali Jamil, Razieh, Hamidi-Fard, Mojtaba, Rahimi, Pooneh, Momen, Seyed Bahman, Aghasadeghi, Mohammad Reza, Allahbakhshi, Elham |
| المصدر: | Scientific Reports ; volume 6, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2016 |
| الوصف: | HIV-1 Vpr is an accessory protein that induces proteasomal degradation of multiple proteins. We recently showed that Vpr targets class I HDACs on chromatin for proteasomal degradation. Here we show that Vpr induces degradation of HDAC1 and HDAC3 in HIV-1 latently infected J-Lat cells. Degradation of HDAC1 and HDAC3 was also observed on the HIV-1 LTR and as a result, markers of active transcription were recruited to the viral promoter and induced viral activation. Knockdown of HDAC1 and HDAC3 activated the latent HIV-1 provirus and complementation with HDAC3 inhibited Vpr-induced HIV-1 reactivation. Viral reactivation and degradation of HDAC1 and HDAC3 was conserved among Vpr proteins of HV-1 group M. Serum Vpr isolated from patients or the release of virion-incorporated Vpr from viral lysates also activated HIV-1 in latently infected cell lines and PBMCs from HIV-1 infected patients. Our results indicate that Vpr counteracts HIV-1 latency by inducing proteasomal degradation of HDAC1 and 3 leading to reactivation of the viral promoter. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/srep31924 |
| الاتاحة: | http://dx.doi.org/10.1038/srep31924 https://www.nature.com/articles/srep31924 https://www.nature.com/articles/srep31924.pdf |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.3AEB26DA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/srep31924 |
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