التفاصيل البيبلوغرافية
العنوان: |
A DHHC-type zinc finger protein gene regulates shoot branching in Arabidopsis |
المؤلفون: |
Xiang, Jing, Lin, Jianzhong, Tang, Dongying, Zhou, Bo, Guo, Ming, He, Reqing, Huang, Xingqun, Zhao, Xiaoying, Liu, Xuanming |
المصدر: |
African Journal of Biotechnology; Vol 9, No 45 (2010); 7759-7766 ; 1684-5315 |
بيانات النشر: |
Academic Journals (Kenya) |
سنة النشر: |
2016 |
المجموعة: |
AJOL - African Journals Online |
مصطلحات موضوعية: |
Arabidopsis, DHHC-type zinc finger protein, At5g04270, shoot branching |
الوصف: |
Formation of plant architecture is a complicated biological phenomenon and is influenced by a variety of factors such as genotype, hormone, environment and nutrition. In this study, an activation-tagging mutant, scc10-D (suppressor of cry1cry2) grown in long-day (16-h light/8-h dark) condition showed enhanced shoot branching. The mRNA expression of six genes adjacent to the T-DNA insertion locus were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR), and the transcript level of a DHHC-type zinc finger protein gene, At5g04270, was found to increase markedly in the scc10-D mutant. The At5g04270 gene was then cloned and over-expressed in Arabidopsis. It was found that the At5g04270 over-expression lines had the features of enhanced shoot branching, while the T-DNA mutant of At5g04270 gene, SALK_006515, showed decreased shoot branching when compared to the wild type (WT). These results suggest that At5g04270 plays an important role in regulating shoot branching in Arabidopsis.Key words: Arabidopsis, DHHC-type zinc finger protein, At5g04270, shoot branching. |
نوع الوثيقة: |
article in journal/newspaper |
وصف الملف: |
application/pdf |
اللغة: |
English |
Relation: |
http://www.ajol.info/index.php/ajb/article/view/130465/120046; http://www.ajol.info/index.php/ajb/article/view/130465 |
الاتاحة: |
http://www.ajol.info/index.php/ajb/article/view/130465 |
Rights: |
Copyright for articles published in this journal is retained by the journal. |
رقم الانضمام: |
edsbas.3A95B68D |
قاعدة البيانات: |
BASE |