Academic Journal
Drosophila adult eye model to teach Scanning Electron Microscopy in an undergraduate cell biology laboratory
| العنوان: | Drosophila adult eye model to teach Scanning Electron Microscopy in an undergraduate cell biology laboratory |
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| المؤلفون: | M References: Busto, B Iyengar, A R Campos, Dettman, R W, F R Turner, D Hoyle, E C Raff, M Friedrich, R Godoy-Herrera, M Alarcon, H Caceres, I I Loyola, J L Navarrete, J Vega, Hassan, M Busto, M Lilly, J Carlson, N Scantlebury, R Sajic, Schneuwly, S, M G Burg, C Lending, M H Perdew, W L Pak, S G Sprecher, C Desplan |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://www.ou.edu/journals/dis/DIS92/Tare%20174.pdf. |
| المجموعة: | CiteSeerX |
| الوصف: | The above protocol offers instructors a dynamic teaching exercise that can be altered to accommodate students at a variety of levels, from an introductory genetics course, to an advanced behavioral or developmental genetics course. This teaching protocol gives students an opportunity to gain experience working with Drosophila, a model species widely used in the study of behavior, development, and genetics. The protocol provides students with hands-on experience in the acquisition of scientific data, from the basic level of sample collection and preparation, to the more advanced data analysis and presentation. The protocol also provides the opportunity for direct experience assaying for mutant phenotypes and in more involved versions the possibility of incorporating actual mutagenesis experiments with this behavioral assay. References Abstract We have devised an undergraduate laboratory exercise to study tissue morphology using fruit fly, Drosophila melanogaster, as the model organism. Drosophila can be reared in a cost effective manner in a short period of time. This experiment was a part of the undergraduate curriculum of the cell biology laboratory course aimed to demonstrate the use of Scanning Electron Microscopy (SEM) technique to study the morphology of adult eye of Drosophila. The adult eye of Drosophila is a compound eye, which comprises of 800 unit eyes, and serves as an excellent model for SEM studies. We used flies that were mutant for Lobe (L), eyeless (ey), and pannier (pnr) for our studies. The mutant flies exhibit different morphologies of the adult eye. We employed a modified protocol, which reduces sample preparation steps and makes it practically feasible to complete the protocol in Dros. Inf. Serv. 92 (2009) Teaching Notes 175 assigned time for the cell biology laboratory. The idea of this laboratory exercise is to: (a) familiarize students with the underlying principles of scanning electron microscopy and its application to diverse areas of research, (b) to enable students to sharpen their ... |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1088.3285; http://www.ou.edu/journals/dis/DIS92/Tare%20174.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1088.3285 http://www.ou.edu/journals/dis/DIS92/Tare%20174.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.3A544BC2 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |