Academic Journal
Apoptosis induced by caffeic acid phenethyl ester in human oral cancer cell lines: Involvement of Puma and Bax activation
| العنوان: | Apoptosis induced by caffeic acid phenethyl ester in human oral cancer cell lines: Involvement of Puma and Bax activation |
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| المؤلفون: | Yu, Hyun-Ju, Shin, Ji-Ae, Yang, In-Hyoung, Won, Dong-Hoon, Ahn, Chi Hyun, Kwon, Hye-Jeong, Lee, Jeong-Sang, Cho, Nam-Pyo, Kim, Eun-Cheol, Yoon, Hye Jung, Lee, Jae Il, Hong, Seong Doo, Cho, Sung-Dae |
| المساهمون: | Yoon, Hye Jung, Lee, Jae Il, Hong, Seong Doo, Cho, Sung-Dae |
| بيانات النشر: | Pergamon Press Ltd. |
| سنة النشر: | 2018 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | BCL-2 FAMILY, ANTIOXIDANT ACTIVITY, TUMOR XENOGRAFT, KAPPA-B, MITOCHONDRIA, CAPE, OLIGOMERIZATION, SUPPRESSES, INHIBITION, PROTEINS, Caffeic acid phenethyl ester (CAPE), Oral cancer, Apoptosis, Neoplastic cell transformation, Bax activation, Puma |
| الوصف: | Objective: Caffeic acid phenethyl ester (CAPE), a natural honeybee product exhibits a spectrum of biological activities including antimicrobial, anti-inflammatory, antioxidant and antitumor actions. The purpose of this research was to investigate the anticancer potential of CAPE and its molecular mechanism in human oral cancer cell lines (YD15, HSC-4 and HN22 cells). Design: To determine the apoptotic activity of CAPE and identify its molecular targets, trypan blue exclusion assay, soft agar assay, Western blot analysis, DAPI staining, and live/dead assay were performed. Results: CAPE significantly suppressed transformation of neoplastic cells induced by epidermal growth factor (EGF) and 12-O-tetradecanoylphorbol 13-acetate (TPA) without inhibiting growth. CAPE treatment inhibited cell growth, increased the cleavages of caspase-3 and poly (ADP-ribose) polymerase (PARP), and augmented the number of fragmented nuclei in human oral cancer cell lines. CAPE activated Bax protein causing it to undergo a conformational change, translocate to the mitochondrial outer membrane, and oligomere. CAPE also significantly increased Puma expression and interestingly Puma and Bax were co-localized. Conclusion: Overall, these results suggest that CAPE is a potent apoptosis-inducing agent in human oral cancer cell lines. Its action is accompanied by up-regulation of Bax and Puma proteins. ; N ; 1 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0003-9969 |
| Relation: | Archives of Oral Biology, Vol.84, pp.94-99; https://hdl.handle.net/10371/206594; 000416190300014; 2-s2.0-85030179324; 40393 |
| DOI: | 10.1016/j.archoralbio.2017.09.024 |
| الاتاحة: | https://hdl.handle.net/10371/206594 https://doi.org/10.1016/j.archoralbio.2017.09.024 |
| رقم الانضمام: | edsbas.3A12183C |
| قاعدة البيانات: | BASE |
| تدمد: | 00039969 |
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| DOI: | 10.1016/j.archoralbio.2017.09.024 |