Academic Journal
P-574: Hypercholesteremia induces enhanced circulating inflammatory activity
| العنوان: | P-574: Hypercholesteremia induces enhanced circulating inflammatory activity |
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| المؤلفون: | Hurley, David J., Parks, Robert J., Lewis, Stephen J., Graves, Jonathan E. |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2004 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Abstracts |
| الوصف: | Hypercholesteremia induces both functional and structural changes in the cardiovascular system including cardiac concentric remodeling, impairment of the baroreflex and endothelial dysfunction. The importance of inflammatory processes in mediating the effects of cardiovascular risk factors, such as hypercholesteremia, is being increasingly recognized. This study examined whether hypercholesteremia induces a systemic inflammatory responses that could play a role in the development of cardiovascular complications Male Hartley guinea pigs (400–450 g) were fed with (n=6) or without (n=6) a 1% cholesterol diet for 13 weeks. 12 ml of blood in EDTA was collected from a carotid artery catheter placed after anesthesia with ketamine (120 mg\kg ip) and acepromazine (12 mg\kg ip). Spleens were removed for transport in phosphate buffered saline (PBS). Leukocytes were collected from buffy coats after lysis of RBC. Spleens were cut and the leukocytes expressed between a pair of microscope slides. Leukocytes were adjusted to 5x106 cells per ml in either PBS with BSA and 5mM glucose or MEM with 10% fetal bovine serum. Cells in PBS were used to measure phagocytosis of bodipy labeled, killed Staphylococcal or E. coli cells and production of phorbol ester induced radical oxygen species (ROS). Phagocytosis was measured by flow cytometry and ROS by fluorescent conversion of dihydrorhodamine 123. A significant increase in ROS production was observed in leukocytes from blood of cholesterol fed animals relative to both control blood leukocytes and spleen cells from either set of animals (See |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://ajh.oxfordjournals.org/cgi/content/short/17/S1/242A-a; http://dx.doi.org/10.1016/j.amjhyper.2004.03.648 |
| DOI: | 10.1016/j.amjhyper.2004.03.648 |
| الاتاحة: | http://ajh.oxfordjournals.org/cgi/content/short/17/S1/242A-a https://doi.org/10.1016/j.amjhyper.2004.03.648 |
| Rights: | Copyright (C) 2004, American Journal of Hypertension, Ltd. |
| رقم الانضمام: | edsbas.397DEFC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.amjhyper.2004.03.648 |
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