Academic Journal
Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation
| العنوان: | Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation |
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| المؤلفون: | Singh, Anand, Wu, Ping, Ryabichko, Sergey, Raimundo, João, Swan, Michael, Wieschaus, Eric, Gregor, Thomas, Toettcher, Jared |
| المساهمون: | Princeton University, Department of Physics, Princeton University (DPPU), The project was supported by NSF grant PHY-1734030 (T.G.) and CAREER–1750663 (J.E.T.), NIH grants R01GM097275 (T.G.), U01DA047730 (T.G.), U01DK127429 (T.G. and J.E.T.), and T32GM007388 (M.S.), and a Princeton MOL Innovation Award (J.E.T.). |
| المصدر: | ISSN: 2211-1247. |
| بيانات النشر: | HAL CCSD Elsevier Inc |
| سنة النشر: | 2022 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | Bicoid, CP: Cell Biology, Drosophila development, gap gene network, kinetics, live imaging, optogenetics, transcription, MESH: Drosophila Proteins, MESH: Gene Expression Regulation, Developmental, MESH: Homeodomain Proteins, MESH: Optogenetics, MESH: Trans-Activators, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV.BDD]Life Sciences [q-bio]/Development Biology |
| الوصف: | International audience ; Developmental patterning networks are regulated by multiple inputs and feedback connections that rapidly reshape gene expression, limiting the information that can be gained solely from slow genetic perturbations. Here we show that fast optogenetic stimuli, real-time transcriptional reporters, and a simplified genetic background can be combined to reveal the kinetics of gene expression downstream of a developmental transcription factor in vivo. We engineer light-controlled versions of the Bicoid transcription factor and study their effects on downstream gap genes in embryos. Our results recapitulate known relationships, including rapid Bicoid-dependent transcription of giant and hunchback and delayed repression of Krüppel. In addition, we find that the posterior pattern of knirps exhibits a quick but inverted response to Bicoid perturbation, suggesting a noncanonical role for Bicoid in directly suppressing knirps transcription. Acute modulation of transcription factor concentration while recording output gene activity represents a powerful approach for studying developmental gene networks in vivo. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/35320726; hal-03896657; https://hal.archives-ouvertes.fr/hal-03896657; https://hal.archives-ouvertes.fr/hal-03896657/document; https://hal.archives-ouvertes.fr/hal-03896657/file/mmc5.pdf; PUBMED: 35320726; PUBMEDCENTRAL: PMC9019726 |
| DOI: | 10.1016/j.celrep.2022.110543 |
| الاتاحة: | https://hal.archives-ouvertes.fr/hal-03896657 https://hal.archives-ouvertes.fr/hal-03896657/document https://hal.archives-ouvertes.fr/hal-03896657/file/mmc5.pdf https://doi.org/10.1016/j.celrep.2022.110543 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.39549178 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.celrep.2022.110543 |
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