Expression of the mouse metallothionein mutant beta beta-cDNA in the lettuces (Lactuca sativa L.)

التفاصيل البيبلوغرافية
العنوان:	Expression of the mouse metallothionein mutant beta beta-cDNA in the lettuces (Lactuca sativa L.)
المؤلفون:	Zuo, XF, Zhang, YK, Wu, BB, Chang, X, Ru, BG
المساهمون:	Ru, BG (reprint author), Peking Univ, State Key Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China., Peking Univ, State Key Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China.
المصدر:	SCI
بيانات النشر:	科学通报英文版
سنة النشر:	2002
المجموعة:	Peking University Institutional Repository (PKU IR) / 北京大学机构知识库
مصطلحات موضوعية:	metallothionein, domain mutant beta beta gene, zinc content, lettuce, ZINC SUPPLEMENTATION, DEVELOPING-COUNTRIES, CHILDREN, GROWTH
الوصف:	Mouse metallothionein (MT) domain mutant betabeta-cDNA gene has been inserted into the plant expression vector pGPTVd35S which has herbicide (PPT)-resistance gene bar as a selective marker. The chimeric gene was introduced into the lettuce (Lactuca sativa L. cv Salinas 88) by Agrobacterium-mediated transformation. PCR and Southern blot analysis of some putative transformants indicated that the introduced betabeta-cDNA was integrated into lettuce genome and inheritted by sexual reproduction. The expression of betabeta gene in lettuce plants was demonstrated by Northern and Western blot analysis. Meanwhile, the zinc content of the transformed lettuce plants is up to 400 mug/g dry weight, remarkably higher than the control plants. ; Multidisciplinary Sciences ; SCI(E) ; 1 ; ARTICLE ; 7 ; 558-562 ; 47
نوع الوثيقة:	journal/newspaper
اللغة:	English
تدمد:	1001-6538
Relation:	CHINESE SCIENCE BULLETIN.2002,47,(7),558-562.; 764546; http://hdl.handle.net/20.500.11897/388392; WOS:000175219200008
DOI:	10.1360/02tb9128
الاتاحة:	https://hdl.handle.net/20.500.11897/388392 https://doi.org/10.1360/02tb9128
رقم الانضمام:	edsbas.393A7741
قاعدة البيانات:	BASE

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الوصف
تدمد:	10016538
DOI:	10.1360/02tb9128