Conference
Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity
| العنوان: | Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity |
|---|---|
| المؤلفون: | Trivanović, Drenka, Kovačević, Jovana, Arsić, Aleksandra, Vujačić, Marko, Bogosavljević, Nikola, Okić Djordjević, Ivana, Živanović, Milena, Mojsilović, Slavko, Maljković, Mirjana, Jauković, Aleksandra |
| المساهمون: | Morić, Ivana, Đorđević, Valentina |
| المصدر: | 4th Belgrade Bioinformatics Conference |
| بيانات النشر: | Institute of molecular genetics and genetic engineering |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | bone marrow adipose tissue, fatty acids, stem cells, adipogenesis, aging |
| الوصف: | As adipose tissue (AT) undergoes metabolic reprogramming with age, we investigated skeletal site-specific and age-dependent lipid profile of bone marrow adipose tissue (BMAT). Acetabular and femoral BMAT, and gluteofemoral subcutaneous adipose tissue (gfSAT) were obtained from matched osteoarthritis patients. Patients were classified into two groups: younger (≤ 60 years) and aged (>60 years) adults. BMAT and gfSAT were explored by using thin layer/gas chromatography coupled with cellular and molecular assays. Data were interpreted and visualized by applying linear discriminant analysis (LDA) and hierarchical clustering of fatty acid (FA) composition. Statistics was estimated by nonparametric tests and Spearman’s rank correlation. Analyses of total lipids revealed significantly reduced triglyceride content in femoral (fBMAT) than in acetabular BMAT (aBMAT) and gfSAT. Frequencies of spontaneously released saturated palmitic (C16:0) and stearic acids (C18:0) were higher in fBMAT than in aBMAT and gfSAT (p=0.036 and p=0.046, n=8). Cluster heatmap and LDA showed that fBMAT differed to acetabular and gfSAT, while acetabular and gfSAT were more similar in FA profiles. FA profiles of AT depots varied with patient’s age. Contribution of palmitic acid was increased in aged group in all AT depots, while stearic acid declined in aged group in BMAT compartments only. fBMAT cellularity declined with age (r=-0.675, n=14, p=0.037). Additionally, the presence of CD45-CD31-CD34+CD24+ adipogenic progenitor (stem) cells was increased in fBMAT (0.46±0.03%) when compared to aBMAT (0.21±0.01%) depot. Femoral mesenchymal stem cells displayed pronounced adipogenesis comparing to their acetabular counterparts. Our findings suggest that specific lipid profile of fBMAT imposes adipogenic commitment of stem cells within this skeletal site. ; Book of abstract: 4th Belgrade Bioinformatics Conference, June 19-23, 2023 |
| نوع الوثيقة: | conference object |
| اللغة: | English |
| Relation: | info:eu-repo/grantAgreement/MESTD/inst-2020/200015/RS//; https://belbi.bg.ac.rs/; https://imagine.imgge.bg.ac.rs/handle/123456789/1995; https://hdl.handle.net/21.15107/rcub_imagine_1995 |
| الاتاحة: | https://imagine.imgge.bg.ac.rs/handle/123456789/1995 https://belbi.bg.ac.rs/ https://imagine.imgge.bg.ac.rs/bitstream/id/305689/BELBI-Abstracts-final-07072023_1-15,70-71,129_1-16,18.pdf https://hdl.handle.net/21.15107/rcub_imagine_1995 |
| Rights: | openAccess ; ARR ; © 2023 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade |
| رقم الانضمام: | edsbas.3903152A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |