التفاصيل البيبلوغرافية
| العنوان: |
Additional file 2 of APOΕ4 lowers energy expenditure in females and impairs glucose oxidation by increasing flux through aerobic glycolysis |
| المؤلفون: |
Brandon C. Farmer (11396919), Holden C. Williams (11396922), Nicholas A. Devanney (11396925), Margaret A. Piron (11396928), Grant K. Nation (11396931), David J. Carter (11396934), Adeline E. Walsh (11396937), Rebika Khanal (11396940), Lyndsay E. A. Young (6329558), Jude C. Kluemper (11396943), Gabriela Hernandez (9462776), Elizabeth J. Allenger (11396946), Rachel Mooney (11396949), Lesley R. Golden (11396952), Cathryn T. Smith (11396955), J. Anthony Brandon (6311996), Vedant A. Gupta (11396958), Philip A. Kern (11396961), Matthew S. Gentry (208345), Josh M. Morganti (7321622), Ramon C. Sun (11396964), Lance A. Johnson (11396967) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Cell Biology, Genetics, Molecular Biology, Neuroscience, Physiology, Evolutionary Biology, Mental Health, Hematology, Computational Biology, Chemical Sciences not elsewhere classified, APOE, Apolipoprotein E, Aerobic glycolysis, Energy expenditure, Metabolism, Alzheimer’s disease |
| الوصف: |
Additional file 2: Supplemental Table 1. Top 25 genes classifying the eight distinct astrocyte clusters identified in scRNAseq analysis of E3 and E4 mouse brains (clusters visualized in Fig. 1e). Supplemental Table 2. Age, sex, and APOE genotype of cognitively normal individuals according to Ε4 carriage and age cohort (young = 18–39, middle-aged = 40–65). Values represent means +/− (SD). Supplemental Table 3. Clinical characteristics of cognitively unimpaired individuals according to Ε4 carriage and age cohort (young = 18–39, middle-aged = 40–65). Values represent means +/− (SD). Ca, Caucasian; AA, African American; His, Hispanic; A, Asian; BMI, body mass index. Supplemental Table 4. Plasma metabolites of study participants analyzed by gas chromatography – before and after a dietary glucose challenge. Supplemental Table 5. Pre-screening checklist. A response of “yes” to any of the following resulted in exclusion from the study. Supplemental Fig. 1. Cluster cell counts. Number of cells in each graph-based cluster from all cells (a), and astrocytes only (b). Bars represent mean number of cells in each cluster, with the number of E3 cells (circles) and E4 cells (squares) noted by symbols. Supplemental Fig. 2. APOE expression in single-cells and specific astrocyte clusters. (a) UMAP visualization of E3 (left) and E4 (right) cells showing expression of APOE. APOE expression is primarily limited to cells identified as astrocytes. (b) Expression of APOE in astrocyte-only UMAP (Aldoc + cells). Inset shows the 8 distinct astrocyte clusters. (c) Violin plots showing expression of APOE in all astrocytes (left) and within each astrocyte cluster (right). (**P < 0.01, ***P < 0.001, unpaired t-test, two-tailed). Supplemental Fig. 3. E4 is associated with decreases in many genes of the oxidative phosphorylation KEGG pathway. Pathway map for KEGG pathway “Oxidative Phosphorylation” showing genes differentially expressed between E3 and E4 astrocytes. Genes highlighted in green are downregulated in E4, genes in red are ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Additional_file_2_of_APO_4_lowers_energy_expenditure_in_females_and_impairs_glucose_oxidation_by_increasing_flux_through_aerobic_glycolysis/16575414 |
| DOI: |
10.6084/m9.figshare.16575414.v1 |
| الاتاحة: |
https://doi.org/10.6084/m9.figshare.16575414.v1 |
| Rights: |
CC BY + CC0 |
| رقم الانضمام: |
edsbas.38C5769 |
| قاعدة البيانات: |
BASE |