Academic Journal
Quick detection of a rare species: forensic swabs of survey tubes for hazel dormouse Muscardinus avellanarius urine
| العنوان: | Quick detection of a rare species: forensic swabs of survey tubes for hazel dormouse Muscardinus avellanarius urine |
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| المؤلفون: | Savolainen, V, Allen, R, Binstead, M, Arnold, R, Priestley, V |
| المصدر: | 827 ; 818 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2021 |
| المجموعة: | Imperial College London: Spiral |
| مصطلحات موضوعية: | Science & Technology, Life Sciences & Biomedicine, Ecology, Environmental Sciences & Ecology, ecological survey, environmental DNA, forensic swab, hazel dormouse, nest tubes, quantitative PCR, rare mammal, species detection, 0502 Environmental Science and Management, 0602 Ecology, 0603 Evolutionary Biology |
| الوصف: | 1. Effective conservation decisions rely on accurate survey data, but methods can be resource‐intensive and risk false negative results. Presence of the threatened hazel dormouse (England, UK) is typically confirmed by looking for its nest in survey tubes, over a 6‐month period. As an alternative, environmental DNA (eDNA) surveys have proven benefits in efficiency and accuracy for other taxa, but generally rely on the extraction and amplification of DNA from water, soil or sediment, which are not yet dependable samples for rare terrestrial mammals like the hazel dormouse. 2. At a known occupancy site, paper‐lined survey tubes were used to capture a DNA sample. Like other species of rodent, the hazel dormouse excretes urine freely, and this was highlighted by ultraviolet torch, swabbed from the paper, extracted and hazel dormouse eDNA amplified by quantitative polymerase chain reaction (qPCR). 3. Hazel dormouse presence was confirmed in this way in three out of 50 tubes within 8 days. Detection by conventional nest survey occurred on day 63 when a hazel dormouse nest was found in a single survey tube. We calculate that amplification of eDNA left behind in tubes increased survey efficiency here at least 12‐fold. 4. Synthesis and applications. In this study we demonstrate that eDNA swabbed from a clean substrate placed in survey apparatus can significantly hasten the detection of a rare species. This method has the potential to broaden the application of eDNA to other terrestrial vertebrates, including surveys at large spatiotemporal scales. Beyond presence/absence, the non‐invasive DNA sample could also offer insights into sex ratio, abundance, behaviour and population genetics. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 2041-210X |
| Relation: | Methods in Ecology and Evolution; http://hdl.handle.net/10044/1/87542 |
| DOI: | 10.1111/2041-210X.13573 |
| الاتاحة: | http://hdl.handle.net/10044/1/87542 https://doi.org/10.1111/2041-210X.13573 |
| Rights: | © 2021 The Authors. Methods in Ecology and Evolution published by John Wiley & Sons Ltd on behalf of British Ecological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.389E53CD |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 2041210X |
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| DOI: | 10.1111/2041-210X.13573 |