Academic Journal
Plant-produced viral nanoparticles as a functionalized catalytic support for metabolic engineering
| العنوان: | Plant-produced viral nanoparticles as a functionalized catalytic support for metabolic engineering |
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| المؤلفون: | Sator, Christian, Lico, Chiara, Pannucci, Elisa, Marchetti, Luca, Baschieri, Selene, Warzecha, Heribert, Santi, Luca |
| المساهمون: | Sator, Christian, Lico, Chiara, Pannucci, Elisa, Marchetti, Luca, Baschieri, Selene, Warzecha, Heribert, Santi, Luca |
| بيانات النشر: | MDPI Basel |
| سنة النشر: | 2024 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| مصطلحات موضوعية: | plant metabolic engineering, plant virus nanoparticle, plant-produced virus-like particle, multi-enzymatic assemblie, olivetolic acid, cannabinoids |
| الوصف: | Substrate channeling could be very useful for plant metabolic engineering; hence, we propose that functionalized supramolecular self-assembly scaffolds can act as enzymatic hubs able to perform reactions in close contiguity. Virus nanoparticles (VNPs) offer an opportunity in this context, and we present a functionalization strategy to display different enzymes on the outer surface of three different VNPs produced in plants. Tomato bushy stunt virus (TBSV) and Potato virus X (PVX) plant viruses were functionalized by the genetic fusion of the E-coil peptide coding sequence to their respective coat proteins genes, while the enzyme lichenase was tagged with the K-coil peptide. Immobilized E-coil VNPs were able to interact in vitro with the plant-produced functionalized lichenase, and catalysis was demonstrated by employing a lichenase assay. To prove this concept in planta, the Hepatitis B core (HBc) virus-like particles (VLPs) were similarly functionalized by genetic fusion with the E-coil sequence, while acyl-activating enzyme 1, olivetolic acid synthase, and olivetolic acid cyclase enzymes were tagged with the K-coil. The transient co-expression of the K-coil-enzymes together with E-coil-VLPs allowed the establishment of the heterologous cannabinoid precursor biosynthetic pathway. Noteworthy, a significantly higher yield of olivetolic acid glucoside was achieved when the scaffold E-coil-VLPs were employed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38498408; info:eu-repo/semantics/altIdentifier/wos/WOS:001172708000001; volume:13; issue:4; numberofpages:14; journal:PLANTS; https://hdl.handle.net/11573/1722720 |
| DOI: | 10.3390/plants13040503 |
| الاتاحة: | https://hdl.handle.net/11573/1722720 https://doi.org/10.3390/plants13040503 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.38391C74 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/plants13040503 |
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