Academic Journal
Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum
| العنوان: | Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum |
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| المؤلفون: | Kubiasova, Karolina, Montesinos López, Juan C, Šamajová, Olga, Nisler, Jaroslav, Mik, Václav, Semeradova, Hana, Plíhalová, Lucie, Novák, Ondřej, Marhavý, Peter, Cavallari, Nicola, Zalabák, David, Berka, Karel, Doležal, Karel, Galuszka, Petr, Šamaj, Jozef, Strnad, Miroslav, Benková, Eva, Plíhal, Ondřej, Spíchal, Lukáš |
| المصدر: | Kubiasova K, Montesinos López JC, Šamajová O, et al. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications . 2020;11. doi: 10.1038/s41467-020-17949-0 |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2020 |
| المجموعة: | IST Austria Research Explorer (Institute of Science and Technology) |
| مصطلحات موضوعية: | ddc:580 |
| الوصف: | Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/issn/20411723; info:eu-repo/semantics/altIdentifier/wos/000567931000002; info:eu-repo/semantics/altIdentifier/pmid/32855390; info:eu-repo/grantAgreement/EC/FP7/291734; info:eu-repo/grantAgreement/FWF//24746; info:eu-repo/grantAgreement/FWF//ALTF710-2016 |
| الاتاحة: | https://research-explorer.ista.ac.at/record/8336 https://research-explorer.ista.ac.at/download/8336/8357 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.3832140F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |