التفاصيل البيبلوغرافية
| العنوان: |
Image_2_A Sequence Type 23 Hypervirulent Klebsiella pneumoniae Strain Presenting Carbapenem Resistance by Acquiring an IncP1 blaKPC-2 Plasmid.tif |
| المؤلفون: |
Rushuang Yan (10902327), Ye Lu (138787), Yiwei Zhu (1419013), Peng Lan (5699819), Shengnan Jiang (7911449), Jun Lu (36049), Ping Shen (149355), Yunsong Yu (496103), Jiancang Zhou (3233460), Yan Jiang (12139) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Clinical Microbiology, Medical Bacteriology, Medical Infection Agents (incl. Prions), Medical Virology, KPC-2, hypervirulent, K. pneumoniae, IncP1 plasmid, P. aeruginosa |
| الوصف: |
Hypervirulent Klebsiella pneumoniae strains are typically associated with severe infections and susceptible to most antimicrobial agents. In 2017, a carbapenem-resistant hypervirulent K. pneumoniae (CR-hvKP) strain was isolated from the sputum of a chronic obstructive pulmonary disease (COPD) patient in Zhejiang, China. The goal of the present study was to characterize the molecular features of the CR-hvKP isolate ZJ27003 and its bla KPC-2 -harboring plasmid p27003_KPC. Antimicrobial susceptibility was evaluated using the broth microdilution and agar dilution methods. String tests, serum-killing and mouse survival assays were performed to assess virulence, and plasmid conjugation was performed by filter mating. The complete genome sequence of ZJ27003 was acquired using a hybrid assembly of Illumina and Nanopore platform data. The sequence type (ST) of this CR-hvKP isolate was identified as ST23, which exhibits hypervirulence with high serum resistance and murine infection model. The strain is also resistant to carbapenems (imipenem, meropenem and ertapenem), aztreonam and cephalosporins. Additionally, the CR-hvKP isolate carries a 36,708-bp bla KPC-2 -harboring plasmid, named p27003_KPC, belonging to the P1 incompatibility (Inc) group. The backbone of p27003_KPC is similar to that of a bla GES-5 -harboring Pseudomonas aeruginosa plasmid, in which the bla GES-5 and its surrounding regions were replaced by a bla KPC-2 -containing translocatable unit derived from Enterobacteriaceae. The results of a conjugation assay revealed that p27003_KPC can be transferred from K. pneumoniae to P. aeruginosa PAO1 and make the recipient resistant against carbapenem. The identification of a carbapenem-resistant hypervirulent K. pneumoniae isolate carrying and disseminating the bla KPC-2 gene highlights a severe threat to public health. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Image_2_A_Sequence_Type_23_Hypervirulent_Klebsiella_pneumoniae_Strain_Presenting_Carbapenem_Resistance_by_Acquiring_an_IncP1_blaKPC-2_Plasmid_tif/14708589 |
| DOI: |
10.3389/fcimb.2021.641830.s002 |
| الاتاحة: |
https://doi.org/10.3389/fcimb.2021.641830.s002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.37204E45 |
| قاعدة البيانات: |
BASE |