Academic Journal
Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2- and Ca 2+ -dependent priming ; Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2- and Ca2+-dependent priming
| العنوان: | Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2- and Ca 2+ -dependent priming ; Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2- and Ca2+-dependent priming |
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| المؤلفون: | Tawfik, B., Martins, J., Houy, S., Imig, C., Pinheiro, P., Wojcik, S., Brose, N., Cooper, B., Sørensen, J. |
| المصدر: | eLife |
| سنة النشر: | 2021 |
| المجموعة: | Max Planck Society: MPG.PuRe |
| الوصف: | Synaptotagmins confer calcium-dependence to the exocytosis of secretory vesicles, but how coexpressed synaptotagmins interact remains unclear. We find that synaptotagmin-1 and synaptotagmin-7 when present alone act as standalone fast and slow Ca2+-sensors for vesicle fusion in mouse chromaffin cells. When present together, synaptotagmin-1 and synaptotagmin-7 are found in largely non-overlapping clusters on dense-core vesicles. Synaptotagmin-7 stimulates Ca2+-dependent vesicle priming and inhibits depriming, and it promotes ubMunc13-2- and phorbolester-dependent priming, especially at low resting calcium concentrations. The priming effect of synaptotagmin-7 increases the number of vesicles fusing via synaptotagmin-1, while negatively affecting their fusion speed, indicating both synergistic and competitive interactions between synaptotagmins. Synaptotagmin-7 places vesicles in close membrane apposition (<6 nm); without it, vesicles accumulate out of reach of the fusion complex (20–40 nm). We suggest that a synaptotagmin-7-dependent movement toward the membrane is involved in Munc13-2/phorbolester/Ca2+-dependent priming as a prelude to fast and slow exocytosis triggering. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://hdl.handle.net/21.11116/0000-000F-5A4A-A; http://hdl.handle.net/21.11116/0000-000F-5A4C-8 |
| الاتاحة: | http://hdl.handle.net/21.11116/0000-000F-5A4A-A http://hdl.handle.net/21.11116/0000-000F-5A4C-8 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.36422EBC |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |