Academic Journal
The effect of anakinra to nephrotoxicity with cisplatin induced in rats: Biochemical, gene expression and histopathological evaluation
| العنوان: | The effect of anakinra to nephrotoxicity with cisplatin induced in rats: Biochemical, gene expression and histopathological evaluation |
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| المؤلفون: | Ozcicek,Adalet. Ozcicek, Fatih. Cimen,Ferda Keskin. Mammadov,Renad, Cankaya,Murat. Ezmeci,Talat. Altuner,Durdu . |
| المساهمون: | EBYÜ, Tıp Fakültesi, orcid:0000-0002-6190-5060 |
| بيانات النشر: | Advances in Clinical and Experimental Medicine |
| سنة النشر: | 2018 |
| المجموعة: | Erzincan Binali Yıldırım University Institutional Repository (DSpace@Erzincan) |
| مصطلحات موضوعية: | rats, cisplatin-nephrotoxicity, anakinra |
| Time: | 10.17219/acem/75775 |
| الوصف: | Background. Oxidative stress and interleukin-1 beta (IL-1β) have been reported to play a role in the pathogenesis of nephrotoxicity induced by cisplatin. Objectives. The objective of this study was to investigate the effect of anakinra, which is an IL-1β receptor antagonist, on cisplatin-induced nephrotoxicity in rats, through biochemical, gene expression and histopathological analyses. Material and methods. The study was designed with 4 groups. For 1 week, the control group (C) and the cisplatin (Cis) group received distilled water, while the cisplatin + anakinra 50 (Cis + ANA50) group and the cisplatin + anakinra 100 (Cis + ANA100) group were intraperitoneally administered 50 mg/kg and 100 mg/kg of anakinra, respectively. The Cis, Cis + ANA50 and Cis + ANA100 groups were intraperitoneally injected with a 2.5 mg/kg dose of cisplatin for 7 days. After sacrifice, the kidney tissue of each rat was extracted for the assessment of the malondialdehyde (MDA) and total glutathione (tGSH) levels, and for gene expression analyses of IL-1β. The kidney tissues were histopathologically evaluated. Statistical analyses of the data were performed using one-way analysis of variance (ANOVA). Results. The administration of cisplatin (the Cis group) yielded a higher level of MDA (4.75 ±0.25 nmol/mL; p < 0.001) and lower levels of tGSH (1.80 ±0.35 mg/L; p < 0.001) compared to other groups. Cisplatin also increased IL-1β gene expression (6.33 ±0.27 gene expression levels; p < 0.001) compared to other groups. The impact of anakinra on the MDA and tGSH levels, and on IL-1β gene expression induced by cisplatin was observed as a reversal of these findings (p < 0.05). Anakinra better prevented an increase of the levels of MDA and IL-1β at a dose of 100 mg/kg compared to a 50 mg/kg dose. Conclusions. Anakinra prevents oxidative kidney damage induced by cisplatin in a dose-dependent manner. This result suggests that anakinra may be useful in the treatment of cisplatin-induced kidney damage |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Turkish |
| تدمد: | 2451-2680 |
| Relation: | Advances in Clinical and Experimental Medicine; Makale - Uluslararası - Editör Denetimli Dergi; https://hdl.handle.net/20.500.12432/2791; 27; 12; 1643; 1650 |
| DOI: | 10.17219/acem/75775 |
| الاتاحة: | https://hdl.handle.net/20.500.12432/2791 https://doi.org/10.17219/acem/75775 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.36312794 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 24512680 |
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| DOI: | 10.17219/acem/75775 |