Dissertation/ Thesis
Functional analysis of the conserved mitochondrial protein Mco76
| العنوان: | Functional analysis of the conserved mitochondrial protein Mco76 |
|---|---|
| المؤلفون: | Khosravi, Siavash |
| بيانات النشر: | Ludwig-Maximilians-Universität München |
| سنة النشر: | 2022 |
| المجموعة: | Electronic Theses of LMU Munich (Ludwig-Maximilians-Universität) |
| مصطلحات موضوعية: | Fakultät für Biologie, ddc:500, ddc:570 |
| الوصف: | Mitochondria execute a multitude of functions in the cell. To maintain their form and function, they need to form a complex membrane system composed of the mitochondrial outer membrane and the mitochondrial inner membrane. Contact sites between mitochondria and other organelles or between the inner mitochondrial membrane (IM) and the outer mitochondrial membrane (OM) are crucial for biogenesis and maintaining the mitochondrial membrane systems. These contacts sites are protein complexes that facilitate the transport of phospholipids and metabolites from other organelles to mitochondria and between mitochondrial membranes. Phospholipids reached to the mitochondria will be further directed to the proper submitochondrial localization, where they will be used for downstream processes. Mco76 is a member of the UbiB protein family containing an ACDK-like kinase (ATPase) domain. Interestingly, Mco76 is conserved throughout the evolution from yeast to human. The study presented here describes a functional analysis of Mco76 by studying its genetic interactions and biochemical interactions. In addition, it provides new insights into the function of Mco76 in the transfer of lipids between the IM and the OM. In this study, evidence was provided that Mco76 is present in the IM of mitochondria while its C-terminus is located in the mitochondrial intermembrane space (IMS). An immunoprecipitation assay was deployed to investigate biochemical interactions of Mco76. For the first time, Por1, Om14, and Om45 from the OM were identified as the protein interactors of Mco76. Hence, it was concluded that a novel contact site between the mitochondrial inner and outer membranes was discovered, providing potential hints into the function of Mco76. The genetic interactions of MCO76 were investigated to understand the role of Mco76 and the contact site formed by its interactions. Deleting of MCO76 from cells in which the CL pathway is impaired leads to a growth phenotype indicating MCO76 plays a role in phospholipid homoeostasis. Although ... |
| نوع الوثيقة: | thesis |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | https://edoc.ub.uni-muenchen.de/30505/; Khosravi, Siavash (2022): Functional analysis of the conserved mitochondrial protein Mco76. Dissertation, LMU München: Fakultät für Biologie |
| الاتاحة: | https://edoc.ub.uni-muenchen.de/30505/1/Khosravi_Siavash.pdf http://nbn-resolving.de/urn:nbn:de:bvb:19-305057 |
| رقم الانضمام: | edsbas.3629E19 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |