Academic Journal
Whole-genome characterisation of Escherichia coli isolates from patients with bacteraemia presenting with sepsis or septic shock in Spain: a multicentre cross-sectional study
| العنوان: | Whole-genome characterisation of Escherichia coli isolates from patients with bacteraemia presenting with sepsis or septic shock in Spain: a multicentre cross-sectional study |
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| المؤلفون: | Maldonado, Natalia, López-Hernández, Inmaculada, García-Montaner, Andrea, López-Cortés, Luis Eduardo, Martínez Pérez-Crespo, Pedro María, Retamar-Gentil, Pilar, Sousa-Domínguez, Adrián, Goikoetxea, Josune, Pulido-Navazo, Ángeles, Labayru-Echevarría, Cristina, Natera-Kindelán, Clara, Jover-Sáenz, Alfredo, Arco-Jiménez, Alfonso del, Armiñanzas Castillo, Carlos, Aller, Ana Isabel, Fernández-Suárez, Jonathan, Smithson-Amat, Alejandro, Reguera-Iglesias, José M., Galán-Sánchez, Fátima, Bahamonde, Alberto |
| المساهمون: | Universidad de Cantabria |
| المصدر: | The Lancet Microbe, 2024, 5, e390-99 |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2024 |
| المجموعة: | Universidad de Cantabria: UCrea |
| الوصف: | Background: Escherichia coli is the most frequent cause of bloodstream infections (BSIs). About one-third of patients with BSIs due to E coli develop sepsis or shock. The objective of this study is to characterise the microbiological features of E coli blood isolates causing sepsis or septic shock to provide exploratory information for future diagnostic, preventive, or therapeutic interventions. Methods: E coli blood isolates from a multicentre cross-sectional study of patients older than 14 years presenting with sepsis or septic shock (according to the Third International Consensus Definitions for Sepsis and Septic Shock criteria) from hospitals in Spain between Oct 4, 2016, and Oct 15, 2017, were studied by whole-genome sequencing. Phylogroups, sequence types (STs), serotype, FimH types, antimicrobial resistance (AMR) genes, pathogenicity islands, and virulence factors were identified. Susceptibility testing was performed by broth microdilution. The main outcome of this study was the characterisation of the E coli blood isolates in terms of population structure by phylogroups, groups (group 1: phylogroups B2, F, and G; group 2: A, B1, and C; group 3: D), and STs and distribution by geographical location and bloodstream infection source. Other outcomes were virulence score and prevalence of virulence-associated genes, pathogenicity islands, AMR, and AMR-associated genes. Frequencies were compared using χ² or Fisher's exact tests, and continuous variables using the Mann-Whitney test, with Bonferroni correction for multiple comparisons. Findings: We analysed 224 isolates: 140 isolates (63%) were included in phylogenetic group 1, 52 (23%) in group 2, and 32 (14%) in group 3. 85 STs were identified, with four comprising 44% (n=98) of the isolates: ST131 (38 [17%]), ST73 (25 [11%]), ST69 (23 [10%]), and ST95 (12 [5%]). No significant differences in phylogroup or ST distribution were found according to geographical areas or source of bloodstream infection, except for ST95, which was more frequent in urinary tract ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2666-5247 |
| Relation: | https://hdl.handle.net/10902/32936 |
| DOI: | 10.1016/S2666-5247(23)00369-5 |
| الاتاحة: | https://hdl.handle.net/10902/32936 https://doi.org/10.1016/S2666-5247(23)00369-5 |
| Rights: | © 2023 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license. ; http://creativecommons.org/licenses/by-nc-nd/4.0/ ; openAccess |
| رقم الانضمام: | edsbas.360AAB43 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 26665247 |
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| DOI: | 10.1016/S2666-5247(23)00369-5 |