Academic Journal
Molecularly imprinted electrochemical biosensor based on Fe@Au nanoparticles involved in 2-aminoethanethiol functionalized multi-walled carbon nanotubes for sensitive determination of cefexime in human plasma
| العنوان: | Molecularly imprinted electrochemical biosensor based on Fe@Au nanoparticles involved in 2-aminoethanethiol functionalized multi-walled carbon nanotubes for sensitive determination of cefexime in human plasma |
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| المؤلفون: | Yola, M.L., Eren, T., Atar, Necip |
| بيانات النشر: | Elsevier Ltd |
| سنة النشر: | 2014 |
| المجموعة: | Pamukkale University Repository / Pamukkale Üniversitesi Açık Erişim Arşivi |
| مصطلحات موضوعية: | Fe@Au nanoparticles, Cefexime, Human plasma, Molecularly imprinting, Multi-walled carbon nanotubes, Validation, Biosensors, Cyclic voltammetry, Infrared spectroscopy, Multiwalled carbon nanotubes (MWCN), Nanoparticles, Photoelectrons, Plasma (human), Polymer membrane electrodes, Self assembly, X ray photoelectron spectroscopy, Au nanoparticle, Human plasmas, Gold, beta lactam antibiotic, buffer, molecular stability, gold nanoparticle, iron, mercaptamine, multi walled nanotube, phosphate, pyrrole, unclassified drug, carbon nanotube |
| الوصف: | The molecular imprinting technique depends on the molecular recognition. It is a polymerization method around the target molecule. Hence, this technique creates specific cavities in the cross-linked polymeric matrices. In present study, a sensitive imprinted electrochemical biosensor based on Fe@Au nanoparticles (Fe@AuNPs) involved in 2-aminoethanethiol (2-AET) functionalized multi-walled carbon nanotubes (f-MWCNs) modified glassy carbon (GC) electrode was developed for determination of cefexime (CEF). The results of X-ray photoelectron spectroscopy (XPS) and reflection-absorption infrared spectroscopy (RAIRS) confirmed the formation of the developed surfaces. CEF imprinted film was constructed by cyclic voltammetry (CV) for 9 cycles in the presence of 80mM pyrrole in phosphate buffer solution (pH 6.0) containing 20mM CEF. The developed electrochemical biosensor was validated according to the International Conference on Harmonisation (ICH) guideline and found to be linear, sensitive, selective, precise and accurate. The linearity range and the detection limit were obtained as 1.0×10-10-1.0×10-8M and 2.2×10-11M, respectively. The developed CEF imprinted sensor was successfully applied to real samples such as human plasma. In addition, the stability and reproducibility of the prepared molecular imprinted electrode were investigated. The excellent long-term stability and reproducibility of the prepared CEF imprinted electrodes make them attractive in electrochemical sensors. © 2014 Elsevier B.V. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Biosensors and Bioelectronics; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://hdl.handle.net/11499/7354; https://doi.org/10.1016/j.bios.2014.04.045; 60; 277; 285; WOS:000337863900040 |
| DOI: | 10.1016/j.bios.2014.04.045 |
| الاتاحة: | https://hdl.handle.net/11499/7354 https://doi.org/10.1016/j.bios.2014.04.045 |
| Rights: | none |
| رقم الانضمام: | edsbas.36020E0A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.bios.2014.04.045 |
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