Academic Journal
Uncoupling growth and succinic acid production in an industrial Saccharomyces cerevisiae strain
| العنوان: | Uncoupling growth and succinic acid production in an industrial Saccharomyces cerevisiae strain |
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| المؤلفون: | Liu, Yaya, Esen, Osman, Pronk, Jack T., van Gulik, Walter M. |
| المصدر: | Biotechnology and Bioengineering ; volume 118, issue 4, page 1557-1567 ; ISSN 0006-3592 1097-0290 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2021 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | This study explores the relation between biomass‐specific succinic acid (SA) production rate and specific growth rate of an engineered industrial strain of Saccharomyces cerevisiae , with the aim to investigate the extent to which growth and product formation can be uncoupled. Ammonium‐limited aerobic chemostat and retentostat cultures were grown at different specific growth rates under industrially relevant conditions, that is, at a culture pH of 3 and with sparging of a 1:1 CO 2 –air mixture. Biomass‐specific SA production rates decreased asymptotically with decreasing growth rate. At near‐zero growth rates, the engineered strain maintained a stable biomass‐specific SA production rate for over 500 h, with a SA yield on glucose of 0.61 mol mol −1 . These results demonstrate that uncoupling of growth and SA production could indeed be achieved. A linear relation between the biomass‐specific SA production rate and glucose consumption rate indicated the coupling of SA production rate and the flux through primary metabolism. The low culture pH resulted in an increased death rate, which was lowest at near‐zero growth rates. Nevertheless, a significant amount of non‐viable biomass accumulated in the retentostat cultures, thus underlining the importance of improving low‐pH tolerance in further strain development for industrial SA production with S. cerevisiae . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/bit.27672 |
| الاتاحة: | http://dx.doi.org/10.1002/bit.27672 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.27672 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/bit.27672 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.35DC6C21 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/bit.27672 |
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