Academic Journal
Assessment of brain-derived extracellular vesicle enrichment for blood biomarker analysis in age-related neurodegenerative diseases : an international overview
| العنوان: | Assessment of brain-derived extracellular vesicle enrichment for blood biomarker analysis in age-related neurodegenerative diseases : an international overview |
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| المؤلفون: | Badhwar, Amanpreet, Hirschberg, Yaël, Valle-Tamayo, Natalia, Iulita, M. Florencia, Udeh-Momoh, Chinedu T., Matton, Anna, Tarawneh, Rawan M., Rissman, Robert A., Ledreux, Aurelie, Winston, Charisse N., Haqqani, Arsalan S. |
| المساهمون: | International Society to Advance Alzheimer’s Research and Treatment: Biofluid Based Biomarkers Professional Interest Area-Exosome Working Group (ISTAART-BBB-PIA-EWG) |
| المصدر: | 1552-5260 ; Alzheimer's & dementia |
| سنة النشر: | 2024 |
| المجموعة: | IRUA - Institutional Repository van de Universiteit Antwerpen |
| مصطلحات موضوعية: | Human medicine |
| الوصف: | INTRODUCTION Brain-derived extracellular vesicles (BEVs) in blood allows for minimally-invasive investigations of central nervous system (CNS) -specific markers of age-related neurodegenerative diseases (NDDs). Polymer-based EV- and immunoprecipitation (IP)-based BEV-enrichment protocols from blood have gained popularity. We systematically investigated protocol consistency across studies, and determined CNS-specificity of proteins associated with these protocols. METHODSNDD articles investigating BEVs in blood using polymer-based and/or IP-based BEV enrichment protocols were systematically identified, and protocols compared. Proteins used for BEV-enrichment and/or post-enrichment were assessed for CNS- and brain-cell-type-specificity, extracellular domains (ECD+), and presence in EV-databases. RESULTSA total of 82.1% of studies used polymer-based (ExoQuick) EV-enrichment, and 92.3% used L1CAM for IP-based BEV-enrichment. Centrifugation times differed across studies. A total of 26.8% of 82 proteins systematically identified were CNS-specific: 50% ECD+, 77.3% were listed in EV-databases. CONCLUSIONS We identified protocol steps requiring standardization, and recommend additional CNS-specific proteins that can be used for BEV-enrichment or as BEV-biomarkers. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/isi/001245071700001 |
| الاتاحة: | https://hdl.handle.net/10067/2067000151162165141 https://repository.uantwerpen.be/docstore/d:irua:24225 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.35C3094A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |