التفاصيل البيبلوغرافية
| العنوان: |
Table_1_Whole-genome sequencing of SARS-CoV-2 isolates from symptomatic and asymptomatic individuals in Tanzania.xlsx |
| المؤلفون: |
Shabani Ramadhani Mziray (9427494), Marco van Zwetselaar (3514238), Charles C. Kayuki (14327085), Peter M. Mbelele (9614134), Abel N. Makubi (14327088), Alex S. Magesa (14327091), Riziki M. Kisonga (14327094), Tolbert B. Sonda (5917391), Gibson S. Kibiki (9614140), George Githinji (3776689), Scott K. Heysell (8656566), Jaffu O. Chilongola (5917400), Stellah G. Mpagama (14327097) |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Dermatology, Emergency Medicine, Gastroenterology and Hepatology, Geriatrics and Gerontology, Intensive Care, Medical Genetics (excl. Cancer Genetics), Nephrology and Urology, Nuclear Medicine, Orthopaedics, Otorhinolaryngology, Pathology (excl. Oral Pathology), Radiology and Organ Imaging, Foetal Development and Medicine, Obstetrics and Gynaecology, Family Care, Primary Health Care, Medical and Health Sciences not elsewhere classified, COVID-19, SARS-CoV-2, whole-genome sequencing, variants of concern, variant of interest, MinION, Oxford Nanopore Technologies (ONT) |
| الوصف: |
Background Coronavirus Disease-2019 (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) accounts for considerable morbidity and mortality globally. Paucity of SARS-CoV-2 genetic data from Tanzania challenges in-country tracking of the pandemic. We sequenced SARS-CoV-2 isolated in the country to determine circulating strains, mutations and phylogenies and finally enrich international genetic databases especially with sequences from Africa. Methods This cross-sectional study utilized nasopharyngeal swabs of symptomatic and asymptomatic adults with positive polymerase chain reaction tests for COVID-19 from January to May 2021. Viral genomic libraries were prepared using ARTIC nCoV-2019 sequencing protocol version three. Whole-genome sequencing (WGS) was performed using Oxford Nanopore Technologies MinION device. In silico genomic data analysis was done on ARTIC pipeline version 1.2.1 using ARTIC nCoV-2019 bioinformatics protocol version 1.1.0. Results Twenty-nine (42%) out of 69 samples qualified for sequencing based on gel electrophoretic band intensity of multiplex PCR amplicons. Out of 29 isolates, 26 were variants of concern [Beta (n = 22); and Delta (n = 4)]. Other variants included Eta (n = 2) and B.1.530 (n = 1). We found combination of mutations (S: D80A, S: D215G, S: K417N, ORF3a: Q57H, E: P71L) in all Beta variants and absent in other lineages. The B.1.530 lineage carried mutations with very low cumulative global prevalence, these were nsp13:M233I, nsp14:S434G, ORF3a:A99S, S: T22I and S: N164H. The B.1.530 lineage clustered phylogenetically with isolates first reported in south-east Kenya, suggesting regional evolution of SARS-CoV-2. Conclusion We provide evidence of existence of Beta, Delta, Eta variants and a locally evolving lineage (B.1.530) from samples collected in early 2021 in Tanzania. This work provides a model for ongoing WGS surveillance that will be required to inform on emerging and circulating SARS-CoV-2 diversity in Tanzania and East Africa. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Table_1_Whole-genome_sequencing_of_SARS-CoV-2_isolates_from_symptomatic_and_asymptomatic_individuals_in_Tanzania_xlsx/21814311 |
| DOI: |
10.3389/fmed.2022.1034682.s002 |
| الاتاحة: |
https://doi.org/10.3389/fmed.2022.1034682.s002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.3578AAB5 |
| قاعدة البيانات: |
BASE |