Academic Journal

Effect of growth regulators on Stevia rebaudiana Bertoni callus genesis and influence of auxin and proline to steviol glycosides, phenols, flavonoids accumulation, and antioxidant activity In Vitro

التفاصيل البيبلوغرافية
العنوان: Effect of growth regulators on Stevia rebaudiana Bertoni callus genesis and influence of auxin and proline to steviol glycosides, phenols, flavonoids accumulation, and antioxidant activity In Vitro
المؤلفون: Blinstrubienė, Aušra, Burbulis, Natalija, Juškevičiūtė, Neringa, Vaitkevičienė, Nijolė, Žūkienė, Rasa
سنة النشر: 2020
المجموعة: Vytautas Magnus University e-Publication Repository (VMU ePub) / Vytauto Didžiojo universitetas: e. publikacijų talpykla (VDU ePub)
مصطلحات موضوعية: Antioxidant activity, Callus, Flavonoids, Stevia rebaudiana Bertoni, Phenolic compounds, Rebaudioside A, Stevioside, Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1), Agronomija / Agronomy (A001), Biochemija / Biochemistry (N004)
جغرافية الموضوع: CH
الوصف: Stevia is a plant containing many active compounds, but usually propagated by stem cuttings because of low seed-yield-germination ability. The aim of this study was to investigate the impact of plant-growth regulators on stevia callus induction and growth from somatic tissue, as well as to determine the effect α-naphthalene acetic acid (NAA) and proline (PRO) on the amount of stevioside, rebaudioside A, phenols, flavonoids, and antioxidant activity. Stem and leaf segments were inoculated on a Murashige and Skoog (MS) medium supplemented with different concentrations of NAA and 6-benzylaminopurine (BAP) for callus genesis. The amount of steviol glycosides (SGs) was evaluated using high-performance liquid chromatography (HPLC), and the amounts of total phenols, flavonoids, and antioxidant activity by spectrophotometric methods. The highest callus-induction frequency and callus-mass increase were obtained from the leaf explants in MS medium supplemented with 2.0 μM NAA. The highest amount of SGs, phenols, and flavonoids, and stronger antioxidant activity were determined in the cellular compounds of callus from leaf explant. PRO reduced the amount of SGs and flavonoids. The significantly highest amount of total phenolic compounds was obtained in the callus from leaf explants in the medium supplemented with 2.0 µM NAA and 2.0 µM PRO ; Agronomijos fakultetas ; Biochemijos katedra ; Vytauto Didžiojo universitetas
نوع الوثيقة: article in journal/newspaper
وصف الملف: p. 1-15; text/xml; application/pdf
اللغة: English
تدمد: 14203049
Relation: Molecules. Basel : MDPI AG, 2020, vol. 25, no. 12; Science Citation Index Expanded (Web of Science); Chemical Abstracts (CAplus); Biological Abstracts; BIOSIS Previews; Current Contents (Life Sciences); Scopus; VDU02-000063856; https://www.vdu.lt/cris/bitstream/20.500.12259/108523/2/ISSN1420-3049_2020_V_25_12.PG_1-15.pdf; https://hdl.handle.net/20.500.12259/108523; https://doi.org/10.3390/molecules25122759
DOI: 10.3390/molecules25122759
الاتاحة: https://www.vdu.lt/cris/bitstream/20.500.12259/108523/2/ISSN1420-3049_2020_V_25_12.PG_1-15.pdf
https://hdl.handle.net/20.500.12259/108523
https://doi.org/10.3390/molecules25122759
Rights: https://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.35509915
قاعدة البيانات: BASE
الوصف
تدمد:14203049
DOI:10.3390/molecules25122759