Academic Journal
Proteomic Analysis of Lipid Droplets from Caco-2/TC7 Enterocytes Identifies Novel Modulators of Lipid Secretion
العنوان: | Proteomic Analysis of Lipid Droplets from Caco-2/TC7 Enterocytes Identifies Novel Modulators of Lipid Secretion |
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المؤلفون: | Beilstein, Frauke, Bouchoux, Julien, Rousset, Monique, Demignot, Sylvie |
المساهمون: | Laboratoire de Santé Publique et Informatique Médicale (SPIM), Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Pharmacologie Cellulaire et Moléculaire, École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU) |
المصدر: | ISSN: 1932-6203. |
بيانات النشر: | HAL CCSD Public Library of Science |
سنة النشر: | 2013 |
مصطلحات موضوعية: | [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism |
الوصف: | International audience ; In enterocytes, the dynamic accumulation and depletion of triacylglycerol (TAG) in lipid droplets (LD) during fat absorption suggests that cytosolic LD-associated TAG contribute to TAG-rich lipoprotein (TRL) production. To get insight into the mechanisms controlling the storage/secretion balance of TAG, we used as a tool hepatitis C virus core protein, which localizes onto LDs, and thus may modify their protein coat and decrease TRL secretion. We compared the proteome of LD fractions isolated from Caco-2/TC7 enterocytes expressing or not hepatitis C virus core protein by a differential proteomic approach (isobaric tag for relative and absolute quantitation (iTRAQ) labeling coupled with liquid chromatography and tandem mass spectrometry). We identified 42 proteins, 21 being involved in lipid metabolism. Perilipin-2/ADRP, which is suggested to stabilize long term-stored TAG, was enriched in LD fractions isolated from Caco-2/TC7 expressing core protein while perilipin-3/TIP47, which is involved in LD synthesis from newly synthesized TAG, was decreased. Endoplasmic reticulum-associated proteins were strongly decreased, suggesting reduced interactions between LD and endoplasmic reticulum, where TRL assembly occurs. For the first time, we show that 17b-hydroxysteroid dehydrogenase 2 (DHB2), which catalyzes the conversion of 17-keto to 17 b-hydroxysteroids and which was the most highly enriched protein in core expressing cells, is localized to LD and interferes with TAG secretion, probably through its capacity to inactivate testosterone. Overall, we identified potential new players of lipid droplet dynamics, which may be involved in the balance between lipid storage and secretion, and may be altered in enterocytes in pathological conditions such as insulin resistance, type II diabetes and obesity. |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
Relation: | hal-01537240; https://hal.sorbonne-universite.fr/hal-01537240; https://hal.sorbonne-universite.fr/hal-01537240/document; https://hal.sorbonne-universite.fr/hal-01537240/file/journal.pone.0053017.PDF |
DOI: | 10.1371/journal.pone.0053017 |
الاتاحة: | https://hal.sorbonne-universite.fr/hal-01537240 https://hal.sorbonne-universite.fr/hal-01537240/document https://hal.sorbonne-universite.fr/hal-01537240/file/journal.pone.0053017.PDF https://doi.org/10.1371/journal.pone.0053017 |
Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
رقم الانضمام: | edsbas.3547A29A |
قاعدة البيانات: | BASE |
DOI: | 10.1371/journal.pone.0053017 |
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