Academic Journal
Genetically Attenuated Plasmodium berghei Liver Stages Induce Sterile Protracted Protection That Is Mediated by Major Histocompatibility Complex Class I–Dependent Interferon- y –Producing CD8 + T Cells
| العنوان: | Genetically Attenuated Plasmodium berghei Liver Stages Induce Sterile Protracted Protection That Is Mediated by Major Histocompatibility Complex Class I–Dependent Interferon- y –Producing CD8 + T Cells |
|---|---|
| المؤلفون: | Jobe, Ousman, Lumsden, Joanne, Mueller, Ann-Kristin, Williams, Jackie, Silva-Rivera, Hilda, Kappe, Stefan H. I., Schwenk, Robert J., Matuschewski, Kai, Krzych, Urszula |
| المصدر: | US Army Research |
| بيانات النشر: | DigitalCommons@University of Nebraska - Lincoln |
| سنة النشر: | 2007 |
| المجموعة: | University of Nebraska-Lincoln: DigitalCommons@UNL |
| مصطلحات موضوعية: | Operations Research, Systems Engineering and Industrial Engineering |
| الوصف: | At present, radiation-attenuated plasmodia sporozoites (y-spz) is the only vaccine that induces sterile and lasting protection in malaria-naive humans and laboratory rodents. However, g-spz are not without risks. For example, the heterogeneity of the y-spz could explain occasional breakthrough infections. To avoid this possibility, we constructed a double-knockout P. berghei parasite by removing 2 genes, UIS3 and UIS4, that are up-regulated in infective spz.We evaluated the double-knockout Pbuis3(-)/4(-) parasites for protective efficacy and the contribution of CD8+ T cells to protection. Pbuis3(-)/4(-) spz induced sterile and protracted protection in C57BL/6 mice. Protection was linked to CD8+ T cells, given that mice deficient in β2m were not protected. Pbuis3(-)/4(-) spz–immune CD8+ T cells consisted of effector/memory phenotypes and produced interferon-y. On the basis of these observations, we propose that the development of genetically attenuated P. falciparum parasites is warranted for tests in clinical trials as a pre-erythrocytic stage vaccine candidate. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | https://digitalcommons.unl.edu/usarmyresearch/129; https://digitalcommons.unl.edu/context/usarmyresearch/article/1128/viewcontent/Jobe_JID_2007_Genetically_attenuated.pdf |
| الاتاحة: | https://digitalcommons.unl.edu/usarmyresearch/129 https://digitalcommons.unl.edu/context/usarmyresearch/article/1128/viewcontent/Jobe_JID_2007_Genetically_attenuated.pdf |
| رقم الانضمام: | edsbas.352C9AEB |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |