Academic Journal
Minimizing polymerase biases in metabarcoding
| العنوان: | Minimizing polymerase biases in metabarcoding |
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| المؤلفون: | Nichols, Ruth V., Vollmers, Christopher, Newsom, Lee A., Wang, Yue, Heintzman, Peter D., Leighton, McKenna, Green, Richard E., Shapiro, Beth |
| بيانات النشر: | Wiley |
| سنة النشر: | 2018 |
| المجموعة: | University of Tromsø: Munin Open Research Archive |
| مصطلحات موضوعية: | VDP::Matematikk og naturvitenskap: 400::Zoologiske og botaniske fag: 480::Vegetasjonshistorie: 495, VDP::Mathematics and natural scienses: 400::Zoology and botany: 480::Vegetation history: 495, VDP::Matematikk og naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økologi: 488, VDP::Mathematics and natural scienses: 400::Zoology and botany: 480::Ecology: 488, bias, eDNA, environmental DNA, metabarcoding, soil, trnL P6 loop |
| الوصف: | This is the peer reviewed version of the following article: Nichols, R.V., Vollmers, C., Newsom, L.A., Wang, Y., Heintzman, P.D., Leighton, M., . Shapiro, B. (2018). Minimizing polymerase biases in metabarcoding. Molecular Ecology Resources, 18 (5), 927-939, which has been published in final form at https://doi.org/10.1111/1755-0998.12895 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. ; DNA metabarcoding is an increasingly popular method to characterize and quantify biodiversity in environmental samples. Metabarcoding approaches simultaneously amplify a short, variable genomic region, or “barcode,” from a broad taxonomic group via the polymerase chain reaction (PCR), using universal primers that anneal to flanking conserved regions. Results of these experiments are reported as occurrence data, which provide a list of taxa amplified from the sample, or relative abundance data, which measure the relative contribution of each taxon to the overall composition of amplified product. The accuracy of both occurrence and relative abundance estimates can be affected by a variety of biological and technical biases. For example, taxa with larger biomass may be better represented in environmental samples than those with smaller biomass. Here, we explore how polymerase choice, a potential source of technical bias, might influence results in metabarcoding experiments. We compared potential biases of six commercially available polymerases using a combination of mixtures of amplifiable synthetic sequences and real sedimentary DNA extracts. We find that polymerase choice can affect both occurrence and relative abundance estimates and that the main source of this bias appears to be polymerase preference for sequences with specific GC contents. We further recommend an experimental approach for metabarcoding based on results of our synthetic experiments. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1755-098X 1755-0998 |
| Relation: | Molecular Ecology Resources; Nichols, R.V., Vollmers, C., Newsom, L.A., Wang, Y., Heintzman, P.D., Leighton, M., . Shapiro, B. (2018). Minimizing polymerase biases in metabarcoding. Molecular Ecology Resources, 18 (5), 927-939. https://doi.org/10.1111/1755-0998.12895; FRIDAID 1588431; https://hdl.handle.net/10037/14475 |
| DOI: | 10.1111/1755-0998.12895 |
| الاتاحة: | https://hdl.handle.net/10037/14475 https://doi.org/10.1111/1755-0998.12895 |
| Rights: | openAccess |
| رقم الانضمام: | edsbas.352A7FA7 |
| قاعدة البيانات: | BASE |
| تدمد: | 1755098X 17550998 |
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| DOI: | 10.1111/1755-0998.12895 |