التفاصيل البيبلوغرافية
| العنوان: |
MiR-200c-3p regulates the expression of stemness markers. |
| المؤلفون: |
Eleni Anastasiadou (4775625), Simona Ceccarelli (49503), Elena Messina (1967659), Giulia Gerini (4405375), Francesca Megiorni (199949), Paola Pontecorvi (8099690), Simona Camero (3410744), Maria Giuseppina Onesti (11450299), Pankaj Trivedi (330168), Mario Faenza (8710755), Enrico Coscioni (10011408), Giovanni Francesco Nicoletti (9239227), Claudio Napoli (119310), Cinzia Marchese (49506) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Cell Biology, Molecular Biology, Immunology, Developmental Biology, Cancer, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, promising therapeutic tools, passaged multiple times, immune regulatory proteins, late passaged ascs, ascs become senescent, mirna modulates pd, div >< p, derived stem cells, 3p promotes proliferation, 3p maintains stemness, maintains stemness, reduced proliferation, immune regulators, ascs reprogramming, vitro <, transiently expressed, therefore investigated, sufficient amount, renewing phenotype, regenerative medicine, proliferative potential |
| الوصف: |
A. qRT-PCR of miR-200c-3p (i), Nanog (ii) , Oct4 (iii) and Sox2 (iv) in ASCs at P4 transfected with miR-200c-3p mimic. The fold change of miR-200c-3p was normalized to U6 and that of stemness markers to GAPDH. SD± is the mean of three experiments and the p values were calculated with unpaired t test, *p<0.05, ***p<0.001. B. qRT-PCR of miR-200c-3p (i) in ASCs transfected with anti-miR-200c-3p. Nanog (ii) , Oct4 (iii) and Sox2 (iv) expressions were normalized to GAPDH. SD± is the mean of three experiments and the p values were calculated with unpaired t test, **p<0.01. C. CD44 mRNA was detected by qRT-PCR (i) and protein expression of CD44, p-STAT3 and STAT3 was detected by WB (ii) , in mimic-miR-200c-3p transfected ASCs. D. CD44 mRNA was detected by qRT-PCR (i) and protein expression of CD44, p-STAT3 and STAT3 was detected by WB (ii) , in anti-miR-200c-3p transfected ASCs. GAPDH was used as housekeeping for qRT-PCR and β-actin in WBs, as a loading control. SD± is the mean of three experiments and the p values were calculated with unpaired t test, *p<0.05, **p<0.01. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/MiR-200c-3p_regulates_the_expression_of_stemness_markers_/16636978 |
| DOI: |
10.1371/journal.pone.0257070.g003 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0257070.g003 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.35195417 |
| قاعدة البيانات: |
BASE |