التفاصيل البيبلوغرافية
| العنوان: |
Multiscale technologies for human stem cell culture in suspension: applications for hematopoietic and cardiac differentiation |
| المؤلفون: |
Zagallo, Monica |
| المساهمون: |
Conconi, Maria Teresa, Zagallo, Monica |
| بيانات النشر: |
Università degli studi di Padova |
| سنة النشر: |
2011 |
| المجموعة: |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
| مصطلحات موضوعية: |
bioreactor, hematopoietic stem cells, cord blood, oxygen, c-kit, human embryonic stem cells, endogenous factor, hydrogel, cardiac differentiation, Settore ING-IND/24 - Principi di Ingegneria Chimica |
| الوصف: |
Stem cell based therapies have been proposed as promising solutions for the regeneration or replacement of diseased tissues, but their potential benefits are limited mainly by scarse cell availability and clinical concern related to cell quality. Aim of the presented research is to develop technologies for human stem cell culture in order to improve the efficiency and the quality of stem cell products. In particular, a bioeractor with a working volume of 10ml/well and a microstructured hydrogel have been designed to perform cultures of human cord blood-derived hematopoietic stem cells (UCB-derived HSCs) in suspension and for controlling cardiac differentiation of human embryonic stem cell-derived embryoid bodies (hES-EBs), respectively. A general aim is to yield the small volumes involved in the proposed technology to span simultaneously a wide range of cell culture conditions which would have prohibitive costs with volumes of hundreds of milliliters. A particularly promising cell-based theraphy is HSC transplantation. UCB-derived CD34+ cells are a source of HSCs potentially very useful for transplantation. However, the current therapeutic use of HSCs from UCB is limited to pediatric patients because of the low cell count from single unit and the difficulty to control the resulting cell phenotype when cells are expanded in vitro. In this scenario, a stirred bioreactor for the expansion of UCB-derived CD34+ cells has been developed in order to investigate the effects of hypoxia on the expression of stem cell markers, as oxygen is recognized to favor stem cell self-renewal in hematopoiesis. The stirring system has been designed on top of a standard six-well plate to favor continuity with conventional static conditions and transfer of culture protocols. The bioreactor volume (10 ml/well), is suitable for cell expansion and multi-parametric flow cytometry analyses. The stirred system improves homogeneity within the culture volume thus allowing to control the effective O2 concentration in the cell immediate ... |
| نوع الوثيقة: |
doctoral or postdoctoral thesis |
| اللغة: |
English |
| Relation: |
alleditors:Conconi, Maria Teresa; http://hdl.handle.net/11577/3427393 |
| الاتاحة: |
http://hdl.handle.net/11577/3427393 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.3518C4DA |
| قاعدة البيانات: |
BASE |