التفاصيل البيبلوغرافية
| العنوان: |
A Non-Viral Plasmid DNA Delivery System Consisting on a Lysine-Derived Cationic Lipid Mixed with a Fusogenic Lipid |
| المؤلفون: |
Martínez-Negro, M. (María), Sánchez-Arribas, N. (Natalia), Guerrero-Martínez, A. (Andrés), Moyá, M.L. (María Luisa), Tros-de-Ilarduya, C. (Conchita), Mendicuti, F. (Francisco), Aicart, E. (Emilio), Junquera, E. (Elena) |
| بيانات النشر: |
MDPI AG |
| سنة النشر: |
2019 |
| المجموعة: |
dadun - Depósito Académico Digital Universidad de Navarra |
| مصطلحات موضوعية: |
Materias Investigacion::Farmacia::Química farmacéutica, Compaction, Gene delivery, Lipoplex, Lysine-derived cationic lipid, Molecular dynamics, Multilamellar aggregates, Plasmid DNA, Protection, Transfection |
| الوصف: |
The insertion of biocompatible amino acid moieties in non-viral gene nanocarriers is an attractive approach that has been recently gaining interest. In this work, a cationic lipid, consisting of a lysine-derived moiety linked to a C12 chain (LYCl) was combined with a common fusogenic helper lipid (DOPE) and evaluated as a potential vehicle to transfect two plasmid DNAs (encoding green fluorescent protein GFP and luciferase) into COS-7 cells. A multidisciplinary approach has been followed: (i) biophysical characterization based on zeta potential, gel electrophoresis, small-angle X-ray scattering (SAXS), and cryo-transmission electronic microscopy (cryo-TEM); (ii) biological studies by fluorescence assisted cell sorting (FACS), luminometry, and cytotoxicity experiments; and (iii) a computational study of the formation of lipid bilayers and their subsequent stabilization with DNA. The results indicate that LYCl/DOPE nanocarriers are capable of compacting the pDNAs and protecting them efficiently against DNase I degradation, by forming Lα lyotropic liquid crystal phases, with an average size of ~200 nm and low polydispersity that facilitate the cellular uptake process. The computational results confirmed that the LYCl/DOPE lipid bilayers are stable and also capable of stabilizing DNA fragments via lipoplex formation, with dimensions consistent with experimental values. The optimum formulations (found at 20% of LYCl content) were able to complete the transfection process efficiently and with high cell viabilities, even improving the outcomes of the positive control Lipo2000*. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://hdl.handle.net/10171/61973 |
| الاتاحة: |
https://hdl.handle.net/10171/61973 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.3467B378 |
| قاعدة البيانات: |
BASE |