Academic Journal
The EspB Protein of Enteropathogenic Escherichia coli Is Targeted to the Cytoplasm of Infected HeLa Cells
| العنوان: | The EspB Protein of Enteropathogenic Escherichia coli Is Targeted to the Cytoplasm of Infected HeLa Cells |
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| المؤلفون: | Taylor, Kathleen A., O’Connell, Colin B., Luther, Paul W., Donnenberg, Michael S. |
| المساهمون: | Orndorff, P. E. |
| المصدر: | Infection and Immunity ; volume 66, issue 11, page 5501-5507 ; ISSN 0019-9567 1098-5522 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 1998 |
| الوصف: | The EspB protein of enteropathogenic Escherichia coli (EPEC) is exported via a type III secretion apparatus. EspB is critical for signaling the host cell and for the development of the attaching and effacing lesion characteristic of EPEC infection. We used cellular fractionation and confocal laser scanning microscopy to determine the cellular location of EspB during infection of HeLa cells. Both methods indicated that EspB is targeted to the cytoplasm of infected cells. Using mutants, we found that EspB targeting to the host cell cytoplasm requires the type III secretion apparatus and the secreted proteins EspA and EspD, but not intimin. These results provide insights into the function of the type III secretion apparatus of EPEC and the functions of the Esp proteins. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/iai.66.11.5501-5507.1998 |
| DOI: | 10.1128/IAI.66.11.5501-5507.1998 |
| الاتاحة: | http://dx.doi.org/10.1128/iai.66.11.5501-5507.1998 https://journals.asm.org/doi/pdf/10.1128/IAI.66.11.5501-5507.1998 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.3454468E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/iai.66.11.5501-5507.1998 |
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