التفاصيل البيبلوغرافية
| العنوان: |
Table_3_Ankk1 Loss of Function Disrupts Dopaminergic Pathways in Zebrafish.pdf |
| المؤلفون: |
Adele Leggieri, Judit García-González, Jose V. Torres-Perez, William Havelange, Saeedeh Hosseinian, Aleksandra M. Mech, Marcus Keatinge, Elisabeth M. Busch-Nentwich, Caroline H. Brennan |
| سنة النشر: |
2022 |
| المجموعة: |
Frontiers: Figshare |
| مصطلحات موضوعية: |
Neuroscience, Biological Engineering, Developmental Biology, Stem Cells, Artificial Intelligence and Image Processing, Endocrinology, Radiology and Organ Imaging, Autonomic Nervous System, Cellular Nervous System, Central Nervous System, Sensory Systems, Clinical Nursing: Tertiary (Rehabilitative), Decision Making, Rehabilitation Engineering, Biomedical Engineering not elsewhere classified, Signal Processing, Neurogenetics, Image Processing, ANKK1, DRD2, dopaminergic system, addiction, amisulpride, apomorphine |
| الوصف: |
Ankyrin repeat and kinase domain containing 1 (ANKK1) is a member of the receptor-interacting protein serine/threonine kinase family, known to be involved in cell proliferation, differentiation and activation of transcription factors. Genetic variation within the ANKK1 locus is suggested to play a role in vulnerability to addictions. However, ANKK1 mechanism of action is still poorly understood. It has been suggested that ANKK1 may affect the development and/or functioning of dopaminergic pathways. To test this hypothesis, we generated a CRISPR-Cas9 loss of function ankk1 zebrafish line causing a 27 bp insertion that disrupts the ankk1 sequence introducing an early stop codon. We found that ankk1 transcript levels were significantly lower in ankk1 mutant (ankk1 27ins ) fish compared to their wild type (ankk1 +/+ ) siblings. In ankk1 +/+ adult zebrafish brain, ankk1 protein was detected in isocortex, hippocampus, basolateral amygdala, mesencephalon, and cerebellum, resembling the mammalian distribution pattern. In contrast, ankk1 protein was reduced in the brain of ankk1 27ins/27ins fish. Quantitative polymerase chain reaction analysis revealed an increase in expression of drd2b mRNA in ankk1 27ins at both larval and adult stages. In ankk1 +/+ adult zebrafish brain, drd2 protein was detected in cerebral cortex, cerebellum, hippocampus, and caudate homolog regions, resembling the pattern in humans. In contrast, drd2 expression was reduced in cortical regions of ankk1 27ins/27ins being predominantly found in the hindbrain. No differences in the number of cell bodies or axonal projections detected by anti-tyrosine hydroxylase immunostaining on 3 days post fertilization (dpf) larvae were found. Behavioral analysis revealed altered sensitivity to effects of both amisulpride and apomorphine on locomotion and startle habituation, consistent with a broad loss of both pre and post synaptic receptors. Ankk1 27ins mutants showed reduced sensitivity to the effect of the selective dopamine receptor antagonist amisulpride on ... |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Table_3_Ankk1_Loss_of_Function_Disrupts_Dopaminergic_Pathways_in_Zebrafish_pdf/19135706 |
| DOI: |
10.3389/fnins.2022.794653.s007 |
| الاتاحة: |
https://doi.org/10.3389/fnins.2022.794653.s007
https://figshare.com/articles/dataset/Table_3_Ankk1_Loss_of_Function_Disrupts_Dopaminergic_Pathways_in_Zebrafish_pdf/19135706 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.342CB2B0 |
| قاعدة البيانات: |
BASE |