Academic Journal
A multiplex PCR assay for the simultaneous detection of Taenia hydatigena, T. multiceps, T. pisiformis, and Dipylidium caninum infections
| العنوان: | A multiplex PCR assay for the simultaneous detection of Taenia hydatigena, T. multiceps, T. pisiformis, and Dipylidium caninum infections |
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| المؤلفون: | Zhu, Guo-Qiang, Li, Li, Ohiolei, John Asekhaen, Wu, Yan-Tao, Li, Wen-Hui, Zhang, Nian-Zhang, Fu, Bao-Quan, Yan, Hong-Bin, Jia, Wan-Zhong |
| المساهمون: | National Key Research and Development Program of China, National Key Basic Research Program (973 Program) of China, Gansu Provincial Key Science and Technology Projects, Science Fund for Creative Research Groups of Gansu Province, Central Public-interest Scientific Institution Basal Research Fund, Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences |
| المصدر: | BMC Infectious Diseases ; volume 19, issue 1 ; ISSN 1471-2334 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2019 |
| الوصف: | Background Taenia hydatigena , T. multiceps , T. pisiformis , and Dipylidium caninum are four common large and medium-sized tapeworms parasitizing the small intestine of dogs and other canids. These parasites cause serious impact on the health and development of livestock. However, there are, so far, no commercially available molecular diagnostic kits capable of simultaneously detecting all four parasites in dogs. The aim of the study was therefore to develop a multiplex PCR assay that will accurately detect all four cestode infections in one reaction. Methods Specific primers for a multiplex PCR were designed based on corresponding mitochondrial genome sequences, and its detection limit was assessed by serial dilutions of the genomic DNAs of tapeworms examined. Furthermore, field samples of dog feces were tested using the developed assay. Results A multiplex polymerase chain reaction (PCR) assay was developed based on mitochondrial DNA ( mt DNA) that accurately and simultaneously identify four cestode species in one reaction using specific fragment sizes of 592, 385, 283, and 190 bp for T. hydatigena , T. multiceps , T. pisiformis , and D. caninum , respectively. The lowest DNA concentration detected was 1 ng for T. hydatigena , T. multiceps and T. pisiformis , and 0.1 ng for D. caninum in a 25 μl reaction system. This assay offers high potential for the rapid detection of these four tapeworms in host feces simultaneously. Conclusions This study provides an efficient tool for the simultaneous detection of T. hydatigena , T. multiceps , T. pisiformis , and D. caninum . The assay will be potentially useful in epidemiological studies, diagnosis, and treatment of these four cestodes infections during prevention and control program. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s12879-019-4512-3 |
| DOI: | 10.1186/s12879-019-4512-3.pdf |
| DOI: | 10.1186/s12879-019-4512-3/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s12879-019-4512-3 http://link.springer.com/content/pdf/10.1186/s12879-019-4512-3.pdf http://link.springer.com/article/10.1186/s12879-019-4512-3/fulltext.html |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.3423753D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12879-019-4512-3 |
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