Academic Journal
Monitoring of intragraft and peripheral blood TIRC7 expression as a diagnostic tool for acute cardiac rejection in humans
| العنوان: | Monitoring of intragraft and peripheral blood TIRC7 expression as a diagnostic tool for acute cardiac rejection in humans |
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| المؤلفون: | Shulzhenko, Natalia UNIFESP, Morgun, Andrey UNIFESP, Rampim, Gisele Fabianne UNIFESP, Franco, Marcello Fabiano de UNIFESP, Almeida, Dirceu Rodrigues de UNIFESP, Diniz, Rosiane Viana Zuza UNIFESP, Carvalho, Antonio Carlos de Camargo UNIFESP, Gerbase-Lima, Maria UNIFESP |
| المساهمون: | Universidade Federal de São Paulo (UNIFESP) |
| بيانات النشر: | Elsevier B.V. |
| سنة النشر: | 2001 |
| المجموعة: | Universidade Federal de São Paulo (UNIFESP): Repositório Institucional |
| مصطلحات موضوعية: | TIRC7, Cardiac allograft rejection, Immunologic monitoring, Gene activation |
| الوصف: | T-cell immune response cDNA 7 (TIRC7) is a recently described T-cell costimulatory molecule that exhibits a central role in T-cell activation in vitro and in vivo. the present study was undertaken to investigate association between intragraft and peripheral blood mononuclear cell (PBMC) TIRC7 mRNA levels and cardiac allograft rejection in humans. TIRC7 gene expression levels were determined by a quantitative-competitive reverse transcriptase-polymerase chain reaction (QC-RT-PCR) in endomyocardial biopsies and in PBMC from cardiac transplant recipients. Biopsies collected during rejection or up to IS days before rejection showed heightened TIRC7 mRNA expression in comparison with biopsies without rejection. All prerejection and rejection biopsies showed TIRC7 mRNA upregulation, while this was present in only 30% of the biopsies without rejection. Regarding TIRC7 mRNA in PBMC, transplant recipients showed lower levels than healthy individuals and, in contrast to the results obtained in biopsies, the levels were lower during rejection than in rejection-free periods. in summary, TIRC7 mRNA expression levels increase in biopsies and decrease in peripheral blood during acute cardiac rejection. We conclude chat intragraft detection of TIRC7 transcripts is a useful tool not only for the diagnosis but also for the prediction of acute heart allograft rejection episodes. (C) American Society for Histocompatibility and Immunogenetics. 2001. Published by Elsevier Science Inc. ; Universidade Federal de São Paulo, Escola Paulista Med, Dept Pediat, BR-04025002 São Paulo, Brazil ; Universidade Federal de São Paulo, Escola Paulista Med, Dept Pathol, BR-04025002 São Paulo, Brazil ; Universidade Federal de São Paulo, Escola Paulista Med, Dept Med, BR-04025002 São Paulo, Brazil ; Universidade Federal de São Paulo, Escola Paulista Med, Dept Pediat, BR-04025002 São Paulo, Brazil ; Universidade Federal de São Paulo, Escola Paulista Med, Dept Pathol, BR-04025002 São Paulo, Brazil ; Universidade Federal de São Paulo, Escola Paulista Med, ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 342-347 |
| اللغة: | English |
| تدمد: | 0198-8859 |
| Relation: | Human Immunology; https://dx.doi.org/10.1016/S0198-8859(01)00211-7; Human Immunology. New York: Elsevier B.V., v. 62, n. 4, p. 342-347, 2001.; https://repositorio.unifesp.br/handle/11600/26519; WOS:000168062900005 |
| DOI: | 10.1016/S0198-8859(01)00211-7 |
| الاتاحة: | https://repositorio.unifesp.br/handle/11600/26519 https://doi.org/10.1016/S0198-8859(01)00211-7 |
| Rights: | Acesso restrito ; http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
| رقم الانضمام: | edsbas.34178B0F |
| قاعدة البيانات: | BASE |
| تدمد: | 01988859 |
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| DOI: | 10.1016/S0198-8859(01)00211-7 |