Academic Journal
Interleukin-6 via Toll-Like Receptor 3 Signaling Attenuates the Expression of Proinflammatory Chemokines in Human Podocytes
| العنوان: | Interleukin-6 via Toll-Like Receptor 3 Signaling Attenuates the Expression of Proinflammatory Chemokines in Human Podocytes |
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| المؤلفون: | Umetsu, Hidenori, Watanabe, Shojiro, Imaizumi, Tadaatsu, Aizawa, Tomomi, Tsugawa, Koji, Kawaguchi, Shogo, Seya, Kazuhiko, Matsumiya, Tomoh, Tanaka, Hiroshi |
| المصدر: | Kidney and Blood Pressure Research ; volume 46, issue 2, page 207-218 ; ISSN 1420-4096 1423-0143 |
| بيانات النشر: | S. Karger AG |
| سنة النشر: | 2021 |
| الوصف: | Background: Although toll-like receptor 3 (TLR3) signaling is involved in the development of certain chronic kidney diseases, the specific molecular mechanisms underlying inflammatory reactions via activation of TLR3 signaling in human podocytes remain unclear. Interleukin (IL)-6 is a pleiotropic cytokine associated with innate and adaptive immune responses; however, little is known about the implication of IL-6 via the activation of regional TLR3 signaling in the inflammatory reactions in human podocytes. Methods: We treated immortalized human podocytes with polyinosinic-polycytidylic acid (poly IC), an authentic viral double-stranded RNA, and assessed the expression of IL-6, monocyte chemoattractant protein-1 (MCP-1), and C-C motif chemokine ligand 5 (CCL5) using quantitative real-time reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. To further elucidate the poly IC-induced signaling pathway, we subjected the cells to RNA interference against IFN-β and IL-6. Results: We found that the activation of TLR3 induced expression of IL-6, MCP-1, CCL5, and IFN-β in human podocytes. RNA interference experiments revealed that IFN-β was involved in the poly IC-induced expression of IL-6, MCP-1, and CCL5. Interestingly, IL-6 knockdown markedly increased the poly IC-induced expression of MCP-1 and CCL5. Further, treatment of cells with IL-6 attenuated the expression of CCL5 and MCP-1 mRNA and proteins. Conclusion: IL-6 induced by TLR3 signaling negatively regulates the expression of representative TLR3 signaling-dependent proinflammatory chemokines in human podocytes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1159/000514589 |
| الاتاحة: | http://dx.doi.org/10.1159/000514589 https://www.karger.com/Article/Pdf/514589 |
| Rights: | https://creativecommons.org/licenses/by-nc/4.0/ ; https://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.340EA67E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1159/000514589 |
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