التفاصيل البيبلوغرافية
| العنوان: |
Functional Analysis of the Fusarielin Biosynthetic Gene Cluster |
| المؤلفون: |
Aida Droce, Wagma Saei, Simon Jørgensen, Reinhard Wimmer, Henriette Giese, Rasmus Wollenberg, Teis Sondergaard, Jens Sørensen |
| المصدر: |
Molecules; Volume 21; Issue 12; Pages: 1710 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2016 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
Fusarium graminearum, trans-enoyl reductase, decalin, polyketide synthases, PKS, secondary metabolites, overexpression, transcription factor, biosynthetic pathway |
| جغرافية الموضوع: |
agris |
| الوصف: |
Fusarielins are polyketides with a decalin core produced by various species of Aspergillus and Fusarium. Although the responsible gene cluster has been identified, the biosynthetic pathway remains to be elucidated. In the present study, members of the gene cluster were deleted individually in a Fusarium graminearum strain overexpressing the local transcription factor. The results suggest that a trans-acting enoyl reductase (FSL5) assists the polyketide synthase FSL1 in biosynthesis of a polyketide product, which is released by hydrolysis by a trans-acting thioesterase (FSL2). Deletion of the epimerase (FSL3) resulted in accumulation of an unstable compound, which could be the released product. A novel compound, named prefusarielin, accumulated in the deletion mutant of the cytochrome P450 monooxygenase FSL4. Unlike the known fusarielins from Fusarium, this compound does not contain oxygenized decalin rings, suggesting that FSL4 is responsible for the oxygenation. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Natural Products Chemistry; https://dx.doi.org/10.3390/molecules21121710 |
| DOI: |
10.3390/molecules21121710 |
| الاتاحة: |
https://doi.org/10.3390/molecules21121710 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.3371E955 |
| قاعدة البيانات: |
BASE |