Academic Journal
Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells
| العنوان: | Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells |
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| المؤلفون: | Pascual-Reguant, Anna, Köhler, Ralf, Mothes, Ronja, Bauherr, Sandy, Hernández, Daniela C., Uecker, Ralf, Holzwarth, Karolin, Kotsch, Katja, Seidl, Maximilian, Philipsen, Lars, Müller, Werner, Romagnani, Chiara, Niesner, Raluca, Hauser, Anja E. |
| سنة النشر: | 2021 |
| المجموعة: | FU Berlin: Refubium |
| مصطلحات موضوعية: | Innate lymphoid cells (ILCs), Multiplexed immunofluorescence technique, Multiparametric histological analysis, ddc:610 |
| الوصف: | Innate lymphoid cells (ILCs) emerge in the last few years as important regulators of immune responses and biological processes. Although ILCs are mainly known as tissue-resident cells, their precise localization and interactions with the microenvironment are still unclear. Here we combine a multiplexed immunofluorescence technique and a customized computational, open-source analysis pipeline to unambiguously identify CD127+ ILCs in situ and characterize these cells and their microenvironments. Moreover, we reveal the transcription factor IRF4 as a marker for tonsillar ILC3, and identify conserved stromal landmarks characteristic for ILC localization. We also show that CD127+ ILCs share tissue niches with plasma cells in the tonsil. Our works thus provide a platform for multiparametric histological analysis of ILCs to improve our understanding of ILC biology. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://refubium.fu-berlin.de/handle/fub188/38463; http://dx.doi.org/10.17169/refubium-38181 |
| DOI: | 10.17169/refubium-38181 |
| DOI: | 10.1038/s41467-021-21994-8 |
| الاتاحة: | https://refubium.fu-berlin.de/handle/fub188/38463 https://doi.org/10.17169/refubium-38181 https://doi.org/10.1038/s41467-021-21994-8 |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.336FA53B |
| قاعدة البيانات: | BASE |
| DOI: | 10.17169/refubium-38181 |
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