التفاصيل البيبلوغرافية
| العنوان: |
A New Method for Isolating and Analysing Coccospheres within Sediment |
| المؤلفون: |
Beth Langley, Paul R. Halloran, Ann Power, Rosalind E. M. Rickaby, Prabhjoat Chana, Poppy Diver, DavidThornalley, Christian Hacker, John Love |
| بيانات النشر: |
Zenodo |
| سنة النشر: |
2020 |
| المجموعة: |
Zenodo |
| الوصف: |
Size is a fundamental cellular trait that is important in determining phytoplankton physiological and ecological processes. Fossil coccospheres, the external calcite structure produced by the excretion of interlocking plates by the phytoplankton coccolithophores, can provide a rare window into cell size in the past. Coccospheres are delicate however and are therefore poorly preserved in sediment. We demonstrate a novel technique combining imaging fow cytometry and cross-polarised light (ISX+PL) to rapidly and reliably visually isolate and quantify the morphological characteristics of coccospheres from marine sediment by exploiting their unique optical and morphological properties. Imaging fow cytometry combines the morphological information provided by microscopy with high sample numbers associated with fow cytometry. High throughput imaging overcomes the constraints of labour-intensive manual microscopy and allows statistically robust analysis of morphological features and coccosphere concentration despite low coccosphere concentrations in sediments. Applying this technique to the fne-fraction of sediments, hundreds of coccospheres can be visually isolated quickly with minimal sample preparation. This approach has the potential to enable rapid processing of downcore sediment records and/or high spatial coverage from surface sediments and may prove valuable in investigating the interplay between climate change and coccolithophore physiological/ecological response. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://zenodo.org/communities/iatlantic-project-collection; https://zenodo.org/communities/eu; oai:zenodo.org:4469322 |
| DOI: |
10.1038/s41598-020-77473-5 |
| الاتاحة: |
https://doi.org/10.1038/s41598-020-77473-5 |
| Rights: |
info:eu-repo/semantics/openAccess ; Creative Commons Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/legalcode |
| رقم الانضمام: |
edsbas.335C61CD |
| قاعدة البيانات: |
BASE |