Academic Journal
Contribution of Membrane Vesicle to Reprogramming of Bacterial Membrane Fluidity in Pseudomonas aeruginosa.
| العنوان: | Contribution of Membrane Vesicle to Reprogramming of Bacterial Membrane Fluidity in Pseudomonas aeruginosa. |
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| المؤلفون: | Mozaheb, Negar, Van Der Smissen, Patrick, Opsomer, Tomas, Mignolet, Eric, Terrasi, Romano, Paquot, Adrien, Larondelle, Yvan, Dehaen, Wim, Muccioli, Giulio, Mingeot-Leclercq, Marie-Paule |
| المساهمون: | UCL - SSS/DDUV - Institut de Duve, UCL - SSS/DDUV/CELL - Biologie cellulaire, UCL - SSS/LDRI - Louvain Drug Research Institute, UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology |
| المصدر: | mSphere, Vol. 7, no. 3, p. e0018722 [1-13] (2022) |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2022 |
| المجموعة: | DIAL@USL-B (Université Saint-Louis, Bruxelles) |
| مصطلحات موضوعية: | P. aeruginosa, Pseudomonas aeruginosa, bacterial membrane, biofilm, biofilms, fluidity, membrane vesicles |
| الوصف: | Pseudomonas aeruginosa is an opportunistic pathogen capable of resisting environmental insults by applying various strategies, including regulating membrane fluidity and producing membrane vesicles (MVs). This study examined the difference in membrane fluidity between planktonic and biofilm modes of growth in P. aeruginosa and whether the ability to alter membrane rigidity in P. aeruginosa could be transferred via MVs. To this end, planktonic and biofilm P. aeruginosa were compared with respect to the lipid composition of their membranes and their MVs and the expression of genes contributing to alteration of membrane fluidity. Additionally, viscosity maps of the bacterial membrane in planktonic and biofilm lifestyles and under the effect of incubation with bacterial MVs were obtained. Further, the growth rate and biofilm formation capability of P. aeruginosa in the presence of MVs were compared. Results showed that the membrane of the biofilm bacteria is significantly less fluid than the membrane of the planktonic bacteria and is enriched with saturated fatty acids. Moreover, the enzymes involved in altering the structure of existing lipids and favoring membrane rigidification are overexpressed in the biofilm bacteria. MVs of biofilm P. aeruginosa elicit membrane rigidification and delay the bacterial growth in the planktonic lifestyle; conversely, they enhance biofilm development in P. aeruginosa. Overall, the study describes the interplay between the planktonic and biofilm bacteria by shedding light on the role of MVs in altering membrane fluidity. Membrane rigidification is a survival strategy in Pseudomonas aeruginosa exposed to stress. Despite various studies dedicated to the mechanism behind this phenomenon, not much attention has been paid to the contribution of the bacterial membrane vesicles (MVs) in this regard. This study revealed that P. aeruginosa rigidifies its membrane in the biofilm mode of growth. Additionally, the capability of decreasing membrane fluidity is transferable to the bacterial ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2379-5042 |
| Relation: | boreal:262731; http://hdl.handle.net/2078.1/262731; info:pmid/35603537; urn:EISSN:2379-5042 |
| DOI: | 10.1128/msphere.00187-22 |
| الاتاحة: | http://hdl.handle.net/2078.1/262731 https://doi.org/10.1128/msphere.00187-22 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.331022E5 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 23795042 |
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| DOI: | 10.1128/msphere.00187-22 |