التفاصيل البيبلوغرافية
| العنوان: |
Biosensor design. |
| المؤلفون: |
Changhui Guan (6261452), Peter J. Larson (14265589), Elizabeth Fleming (217050), Alexander P. Tikhonov (14265592), Sara Mootien (431957), Trudy H. Grossman (1585339), Caroline Golino (14265595), Julia Oh (240419) |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Medicine, Microbiology, Biotechnology, Immunology, Developmental Biology, Cancer, Infectious Diseases, Virology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Mathematical Sciences not elsewhere classified, significant clinical concern, selectively deliver antimicrobials, numerous beneficial functions, engineered skin probiotic, div >< p, prolonged antibiotic exposure, >) aureus <, staphylococcus aureus <, aureus <, antibiotic resistance, vitro <, epidermidis <, “ detect, producing lysostaphin, pathogen colonization, mrsa ), discuss challenges |
| الوصف: |
A) Illustration of the staphylococcal agr quorum-sensing circuit. AgrD pro-peptide is modified and exported by AgrB as an auto-inducer peptide (AIP). AIP stimulates two component sensor AgrCA, which upregulates transcription of the P2/P3 promoter, upregulating agr component and RNAIII expression. B) Concept illustration of a S . epidermidis “detect and destroy” probiotic biosensor. The sensing component from S . aureus’ quorum sensing circuit in A) ( agrA and agrC) are introduced into an agr mutant S . epidermidis strain. Binding of AgrA to the P2/P3 promoter controlling expression of a S . aureus -targeting antimicrobial then results in density-dependent production of the antimicrobial of choice, here, lysostaphin. C) Staphylococcal biosensor circuit design for validation. agrCA sensor genes were cloned from S . aureus agr groups I-IV and placed downstream of promoter P2, with GFP under control of P3. D) Biosensor induction by different AIPs from different S . aureus agr types, testing the efficacy and the cross-reactivity of a plasmid-borne biosensor. Supernatants of overnight S . aureus cultures from each agr type were filter-sterilized, diluted 1:10 in TSB and co-incubated with S . epidermidis strains expressing the corresponding agr biosensor reporter constructs (sensor). Control was empty vector (pCN34). Bidirectional T-test compared to TSB control group, *: p ≤ 0.05, **: p ≤ 0.01, ***: p ≤ 0.001, n = 2 replicates. E) Testing efficacy and sensitivity of biosensor induction by the matched agr type AIP, using a biosensor integrated into the S . epidermidis genome. Given low cross-reactivity from D), we tested each biosensor with its matching agr type in a dilution series to test sensitivity. Biosensors were co-incubated with dilutions of supernatant from S . aureus strains of the matching agr type. Media was used as control. Significance between groups determined within each panel by ANOVA with post-hoc Tukey HSD test, p < 0.05, n = 3 replicates. Groups that do not share the same letter (a, b, c, or d) ... |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Biosensor_design_/21733858 |
| DOI: |
10.1371/journal.pone.0276795.g001 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0276795.g001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.32DCE1F8 |
| قاعدة البيانات: |
BASE |