التفاصيل البيبلوغرافية
| العنوان: |
Method to Investigate the Distribution of Water-Soluble Drug-Delivery Systems in Fresh Frozen Tissues Using Imaging Mass Cytometry |
| المؤلفون: |
Nicole Strittmatter (1646014), Richard M. England (1519039), Alan M. Race (1361004), Daniel Sutton (10090965), Jennifer I. Moss (9179361), Gareth Maglennon (9174374), Stephanie Ling (9174365), Edmond Wong (188316), Jonathan Rose (705648), Ian Purvis (77622), Ruth Macdonald (10170736), Simon T. Barry (9174377), Marianne B. Ashford (8532876), Richard J. A. Goodwin (7439648) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Biotechnology, Space Science, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, tissue structures, S-Dend, tissue staining, imaging acquisitions, IMC experiment, multiplexed experiment, antibody staining, 1 μ m, laser ablation inductively, halogen-containing xenobiotics, LA-ICP-MSI, tissue matrix, ablation step, tissue section, image metal, plasma mass spectrometry, strategy, Water-Soluble Drug-Delivery Systems, IMC analysis, sample-processing steps, workflow results, 2 μ m, cell types |
| الوصف: |
Imaging mass cytometry (IMC) offers the opportunity to image metal- and heavy halogen-containing xenobiotics in a highly multiplexed experiment with other immunochemistry-based reagents to distinguish uptake into different tissue structures or cell types. However, in practice, many xenobiotics are not amenable to this analysis, as any compound which is not bound to the tissue matrix will delocalize during aqueous sample-processing steps required for IMC analysis. Here, we present a strategy to perform IMC experiments on a water-soluble polysarcosine-modified dendrimer drug-delivery system (S-Dends). This strategy involves two consecutive imaging acquisitions on the same tissue section using the same instrumental platform, an initial laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MSI) experiment followed by tissue staining and a standard IMC experiment. We demonstrated that settings can be found for the initial ablation step that leave sufficient residual tissue for subsequent antibody staining and visualization. This workflow results in lateral resolution for the S-Dends of 2 μm followed by imaging of metal-tagged antibodies at 1 μm. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Method_to_Investigate_the_Distribution_of_Water-Soluble_Drug-Delivery_Systems_in_Fresh_Frozen_Tissues_Using_Imaging_Mass_Cytometry/14067109 |
| DOI: |
10.1021/acs.analchem.0c03908.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.analchem.0c03908.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.32D6B9DF |
| قاعدة البيانات: |
BASE |