التفاصيل البيبلوغرافية
| العنوان: |
Molecular Basis for Environment Sensing by a Nucleoid-Structuring Bacterial Protein Filament |
| المؤلفون: |
Xiaochuan Zhao (737365), Jacob M. Remington (10430672), Severin T. Schneebeli (547859), Stefan T. Arold (261112), Jianing Li (359834) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Biotechnology, Ecology, Cancer, Inorganic Chemistry, Computational Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, temperature-inducible strain, insight, histone-like nucleoid, Gram-positive bacteria, simulation, DNA binding, DNA interactions, mechanism, superhelical, Molecular Basis, Protein Filament, use multiscale modeling, environment sensor |
| الوصف: |
The histone-like nucleoid structuring (H‑NS) protein controls the expression of hundreds of genes in Gram-positive bacteria through its capability to coat and condense DNA. This mechanism requires the formation of superhelical H‑NS protein filaments that are sensitive to temperature and salinity, allowing H‑NS to act as an environment sensor. We use multiscale modeling and simulations to obtain detailed insights into the mechanism of H‑NS filament’s sensitivity to environmental changes. Through the simulations of the superhelical H‑NS filament, we reveal how different environments induce heterogeneity of H‑NS monomers. Further, we observe that transient self-association within the H‑NS filament creates temperature-inducible strain and might mildly oppose DNA binding. We also probe different H‑NS–DNA complex architectures and show that complexation enhances the stability of both DNA and H‑NS superhelices. Overall, our results provide unprecedented molecular insights into the environmental sensing and DNA interactions of a prototypical nucleoid-structuring bacterial protein filament. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Molecular_Basis_for_Environment_Sensing_by_a_Nucleoid-Structuring_Bacterial_Protein_Filament/15157039 |
| DOI: |
10.1021/acs.jpclett.1c01710.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.jpclett.1c01710.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.32B222EA |
| قاعدة البيانات: |
BASE |