Academic Journal
Surprising absence of heparin in the intestinal mucosa of baby pigs
| العنوان: | Surprising absence of heparin in the intestinal mucosa of baby pigs |
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| المؤلفون: | Yu, Yanlei, Chen, Yin, Mikael, Paiyz, Zhang, Fuming, Stalcup, Apryll M., German, Rebecca, Gould, Francois, Ohlemacher, Jocelyn, Zhang, Hong, Linhardt, Robert J. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Biology, Chemistry and chemical biology, Chemical and biological engineering, Biomedical engineering |
| الوصف: | Glycobiology, 27, 57–63 ; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. ; Heparin, a member of a family of molecules called glycosaminoglycans, is biosynthesized in mucosal mast cells. This important anticoagulant polysaccharide is primarily produced by extraction of the mast cell-rich intestinal mucosa of hogs. There is concern about our continued ability to supply sufficient heparin to support the worldwide growth of advanced medical procedures from the static population of adult hogs used as food animals. While the intestinal mucosa of adult pigs is rich in anticoagulant heparin (containing a few hundred milligrams per animal), little is known about how the content of heparin changes with animal age. Using sophisticated mass spectral analysis we discovered that heparin was largely absent from the intestinal mucosa of piglets. Moreover, while the related, nonanticoagulant heparan sulfate glycosaminoglycan was present in significant amounts we found little chondroitin sulfate E also associated with mast cells. Histological evaluation of piglet intestinal mucosa showed a very low mast cell content. Respiratory mast cells have been reported in baby pigs suggesting that there was something unique about the piglets used in the current study. These piglets were raised in the relatively clean environment of a university animal facility and treated with antibiotics over their lifetime resulting in a depleted microbiome that greatly reduced the number of mast cells and heparin content of the intestinal mucosal in these animals. Thus, from the current study it remains unclear whether the lack of intestinal mast cell-derived heparin results from the young age of these animals or their exposure to their depleted microbiome. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 14602423 |
| Relation: | The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS); The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Glycobiology; https://harc.rpi.edu/; Surprising absence of heparin in the intestinal mucosa of baby pigs, Y. Yu, Y. Chen, P. Mikael, F. Zhang, A. M. Stalcup, R. German, F. Gould, J. Ohlemacher, H. Zhang, R. J. Linhardt, Glycobiology, 27, 57–63, 2017; 9596658; https://doi.org/10.1093/glycob/cww104; https://hdl.handle.net/20.500.13015/5644 |
| DOI: | 10.1093/glycob/cww104 |
| الاتاحة: | https://hdl.handle.net/20.500.13015/5644 https://doi.org/10.1093/glycob/cww104 |
| Rights: | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ |
| رقم الانضمام: | edsbas.32B10263 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14602423 |
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| DOI: | 10.1093/glycob/cww104 |