Academic Journal
Boosting the therapeutic potential of cell secretome against osteoarthritis: Comparison of cytokine-based priming strategies
| العنوان: | Boosting the therapeutic potential of cell secretome against osteoarthritis: Comparison of cytokine-based priming strategies |
|---|---|
| المؤلفون: | C. Giannasi, E. Della Morte, F. Cadelano, A. Valenza, S. Casati, M. Dei Cas, S. Niada, A. Brini |
| المساهمون: | C. Giannasi, E. Della Morte, F. Cadelano, A. Valenza, S. Casati, M. Dei Ca, S. Niada, A. Brini |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2024 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | Settore BIO/13 - Biologia Applicata, Settore BIO/14 - Farmacologia |
| الوصف: | The secretome, or conditioned medium (CM), from Mesenchymal Stem/stromal Cells (MSCs) has recently emerged as a promising cell-free therapeutic against osteoarthritis (OA), capable of promoting cartilage regen- eration and immunoregulation. Priming MSCs with 10 ng/ml tumor necrosis factor α (TNFα) and/or 10 ng/ml interleukin 1β (IL-1β) aims at mimicking the pathological milieu of OA joints in order to target their secretion towards a pathology-tailored phenotype. Here we compare the composition of the CM obtained after 24 or 72 h from untreated and cytokine-treated adipose-derived MSCs (ASCs). The 72-hour double-primed CM presents a higher total protein yield, a larger number of extracellular vesicles, and a greater concentration of bioactive lipids, in particular sphingolipids, fatty acids, and eicosanoids. Moreover, the levels of several factors involved in immunomodulation and regeneration, such as TGF-β1, PGE2, and CCL-2, are strongly upregulated. Additionally, the differential profiling of 80 bioactive molecules indicates that primed CM is enriched in immune cell chemotaxis and migration factors. Our results indicate that pre-conditioning ASCs with inflammatory cytokines can modulate the composition of their CM, promoting the release of factors with recognized anti-inflammatory, chondroprotective, and immunoregulatory properties. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38042116; info:eu-repo/semantics/altIdentifier/wos/WOS:001133286000001; volume:170; firstpage:1; lastpage:11; numberofpages:11; journal:BIOMEDICINE & PHARMACOTHERAPY; https://hdl.handle.net/2434/1019650; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85179076754 |
| DOI: | 10.1016/j.biopha.2023.115970 |
| الاتاحة: | https://hdl.handle.net/2434/1019650 https://doi.org/10.1016/j.biopha.2023.115970 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.3298B5EA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.biopha.2023.115970 |
|---|