Academic Journal
The Antigenicity of Ex Vivo Cultivated Human Corneal Limbal Epithelial and Stromal Cells: Temporal Changes In Vitro
| العنوان: | The Antigenicity of Ex Vivo Cultivated Human Corneal Limbal Epithelial and Stromal Cells: Temporal Changes In Vitro |
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| المؤلفون: | Oh, Joo Youn, Ko, Jung Hwa, Lee, Hyun Ju, Kim, Mee Kum, Lee, Jin Hak, Wee, Won Ryang |
| المساهمون: | Oh, Joo Youn, Kim, Mee Kum, Wee, Won Ryang |
| بيانات النشر: | Lippincott Williams & Wilkins Ltd. |
| سنة النشر: | 2021 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | OCULAR-SURFACE DISORDERS, ANTERIOR LAMELLAR KERATOPLASTY, HLA-G, PENETRATING KERATOPLASTY, KERATOLIMBAL ALLOGRAFT, STEM-CELLS, TRANSPLANTATION, DEFICIENCY, RECONSTRUCTION, KERATOCONUS, corneal stromal cell, costimulatory molecule, cultivation, human leukocyte antigen, limbal epithelial cell |
| الوصف: | Purpose: To investigate the effect of ex vivo culture on the expression of human leukocyte antigen (HLA) in human corneal limbal epithelial cells (LECs), and to evaluate the expression of HLA and costimulatory molecules on human corneal limbal stromal cells (LSCs). Methods: The expression of HLA-ABC and HLA-DR were evaluated using flow cytometry on cultured human LECs and LSCs after serial passage and compared with that on freshly isolated cells. Additionally, the expression of costimulatory molecules CD80, CD86, CD40, and immunoregulatory molecule HLA-G were investigated on LSCs with or without an addition of interferon (IFN) gamma (250 U/mL, 4 days). Results: HLA-ABC and HLA-DR were expressed in 99.12 +/- 1.02% and 25.86 +/- 4.34% of freshly isolated LECs, respectively. After culture, the HLA-DR-expressing cells significantly decreased with serial passage, whereas the percentage of HLA-ABC-expressing cells did not change. More than 90% of LSCs expressed HLA-ABC and HLA-G, whereas the expression of HLA-DR, CD80, CD86, and CD40 was negligible. This expression profile did not change after serial passage. However, the treatment with IFN-gamma induced a significant increase in HLA-DR (0.17% vs. 17.68%) and CD40 (0.19% vs. 13.19%) expression on LSCs. Conclusions: The immunogenicity of LECs can be lowered after ex vivo cultivation, whereas LSCs can be immunogenic in the presence of IFN-gamma. ; N ; 1 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| ردمك: | 978-0-00-283329-5 0-00-283329-8 |
| تدمد: | 0277-3740 |
| Relation: | Cornea, Vol.29 No.11, pp.1302-1307; https://hdl.handle.net/10371/202968; 000283329800019; 2-s2.0-78149279706; 151464 |
| DOI: | 10.1097/ICO.0b013e3181e3f01e |
| الاتاحة: | https://hdl.handle.net/10371/202968 https://doi.org/10.1097/ICO.0b013e3181e3f01e |
| رقم الانضمام: | edsbas.324426EB |
| قاعدة البيانات: | BASE |
| ردمك: | 9780002833295 0002833298 |
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| تدمد: | 02773740 |
| DOI: | 10.1097/ICO.0b013e3181e3f01e |