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Bernhard Friedrich,1 Rainer Tietze,1 Michaela Dümig,2 Alexandru Sover,3 Marius-Andrei Boca,3 Eveline Schreiber,1 Julia Band,1 Christina Janko,1 Sven Krappmann,2 Christoph Alexiou,1,* Stefan Lyer1,4,* 1Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, Germany; 2Mikrobiologisches Institut – Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany; 3Faculty of Engineering, Ansbach University of Applied Sciences, Ansbach, Germany; 4Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Professorship for AI-Controlled Nanomaterials, Universitätsklinikum Erlangen, Erlangen, Germany*These authors contributed equally to this workCorrespondence: Stefan Lyer, Section of Experimental Oncology & Nanomedicine (SEON), Glückstraße 10a, Erlangen, 91054, Germany, Tel +49-9131-85-33142, Fax +49-9131-85-34828, Email stefan.lyer@uk-erlangen.dePurpose: Magnetic separation of microbes can be an effective tool for pathogen identification and diagnostic applications to reduce the time needed for sample preparation. After peptide functionalization of superparamagnetic iron oxide nanoparticles (SPIONs) with an appropriate interface, they can be used for the separation of sepsis-associated yeasts like Candida albicans. Due to their magnetic properties, the magnetic extraction of the particles in the presence of an external magnetic field ensures the accumulation of the targeted yeast.Materials and Methods: In this study, we used SPIONs coated with 3-aminopropyltriethoxysilane (APTES) and functionalized with a peptide originating from GP340 (SPION-APTES-Pep). For the first time, we investigate whether this system is suitable for the separation and enrichment of Candida albicans, we investigated its physicochemical ... |